The active ingredients analysis of Herba Lysimachiae treating osteoarthritis based on the LC–MS/MS technology and public bioinformatics platforms

Autor: Wu-de Yang, Ying Zhou, Shuai-nan Zhang, Qi Liu, Xu-Zhao Li
Rok vydání: 2021
Předmět:
Zdroj: Journal of Separation Science. 44:3799-3809
ISSN: 1615-9314
1615-9306
DOI: 10.1002/jssc.202100390
Popis: Herba Lysimachiae inhibits synovial damage in osteoarthritis via regulating two biolabels (integrin alpha 2b/beta 3). However, the relevant active ingredients are still unknown. Here, the active ingredients of herbal medicines were analyzed based on the liquid chromatography-tandem mass spectrometry technology and public bioinformatics platforms. The liquid chromatography-tandem mass spectrometry technology was used for compound analysis, and public databases (PubChem BioAssay and STRING) were applied to establish the links between herbal compounds and both biolabels, and identify which herbal compounds may regulate these biolabels. Subsequently, osteoarthritis model was used to confirm the results. Totally, ninety compounds in Herba Lysimachiae were identified based on the liquid chromatography-tandem mass spectrometry technology. Bioinformatics analysis showed that five compounds (myricetin, fisetin, esculetin, 7-hydroxycoumarin-4-acetic acid, and caffeic acid) may synergistically regulate biolabels through 11 targets, which may be the active ingredients of Herba Lysimachiae for osteoarthritis treatment. In the verification experiments, five compounds markedly suppressed the overexpression of biolabels in the synovium of the osteoarthritis model. In conclusion, the present study effectively and rapidly analyzed the active ingredients of Herba Lysimachiae for osteoarthritis treatment. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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