A two-lane mechanism for selective biological ammonium transport
| Autor: | Gaëtan Dias Mirandela, Mélanie Boeckstaens, Anna Maria Marini, Gordon Williamson, Ulrich Zachariae, Arnaud Javelle, Eilidh Terras, Marcus G Bage, Paul A. Hoskisson, Giulia Tamburrino, Andrei V. Pisliakov, Adriana Bizior, Callum M. Ives |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
transport selectivity rhesus protein QH301-705.5 Science Saccharomyces cerevisiae ammonium transporter Chemical biology nitrosomonas europaea 010402 general chemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Molecular dynamics chemistry.chemical_compound Deprotonation Nitrosomonas europaea Molecule General Materials Science Ammonium Biology (General) 030304 developmental biology 0303 health sciences 030102 biochemistry & molecular biology General Immunology and Microbiology biology 030306 microbiology General Neuroscience Substrate (chemistry) Biological membrane General Medicine Sciences bio-médicales et agricoles biology.organism_classification QR 0104 chemical sciences 030104 developmental biology Membrane chemistry Biophysics Medicine SSME Microbiologie et protistologie [bacteriol.virolog.mycolog.] Ammonium transport |
| Zdroj: | eLife, 9 eLife, Vol 9 (2020) |
| ISSN: | 2050-084X |
| Popis: | The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH4+ transport in two archetypal members of the family, the transporters AmtB from Escherichia coli and Rh50 from Nitrosomonas europaea. The pathway underpins a mechanism by which charged H+ and neutral NH3 are carried separately across the membrane after NH4+ deprotonation. This mechanism defines a new principle of achieving transport selectivity against competing ions in a biological transport process. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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