The Jellyfish Rhizostoma pulmo (Cnidaria): Biochemical Composition of Ovaries and Antibacterial Lysozyme-like Activity of the Oocyte Lysate
| Autor: | Lorena Basso, Silvia Lomartire, Federica Angilè, Francesco Paolo Fanizzi, Lucia Rizzo, Loredana Stabili, Stefano Piraino, Chiara Roberta Girelli, Laura Del Coco |
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| Přispěvatelé: | Stabili, Loredana, Rizzo, Lucia, Fanizzi, Francesco Paolo, Angilè, Federica, Coco, Laura Del, Girelli, Chiara Roberta, Lomartire, Silvia, Piraino, Stefano, Basso, Lorena |
| Jazyk: | English, Middle (1100-1500) |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Jellyfish Lysis Pharmaceutical Science Rhizostoma pulmo 01 natural sciences 03 medical and health sciences chemistry.chemical_compound NMR spectroscopy Dry weight antibacterial activity biology.animal Drug Discovery Organic matter 14. Life underwater Food science Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 chemistry.chemical_classification biology Chemistry 010604 marine biology & hydrobiology biochemical characterization biology.organism_classification 030104 developmental biology lcsh:Biology (General) jellyfish blooms Lysozyme Antibacterial activity Polyunsaturated fatty acid |
| Zdroj: | Marine drugs 17 (2019). doi:10.3390/md17010017 info:cnr-pdr/source/autori:Stabili, Loredana; Rizzo, Lucia; Fanizzi, Francesco Paolo; Angile, Federica; Del Coco, Laura; Girelli, Chiara Roberta; Lomartire, Silvia; Piraino, Stefano; Basso, Lorena/titolo:The Jellyfish Rhizostoma pulmo (Cnidaria): Biochemical Composition of Ovaries and Antibacterial Lysozyme-like Activity of the Oocyte Lysate/doi:10.3390%2Fmd17010017/rivista:Marine drugs/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:17 Marine Drugs Volume 17 Issue 1 Marine Drugs, Vol 17, Iss 1, p 17 (2018) |
| DOI: | 10.3390/md17010017 |
| Popis: | Jellyfish outbreaks in marine coastal areas represent an emergent problem worldwide, with negative consequences on human activities and ecosystem functioning. However, potential positive effects of jellyfish biomass proliferation may be envisaged as a natural source of bioactive compounds of pharmaceutical interest. We investigated the biochemical composition of mature female gonads and lysozyme antibacterial activity of oocytes in the Mediterranean barrel jellyfish Rhizostoma pulmo. Chemical characterization was performed by means of multinuclear and multidimensional NMR spectroscopy. The ovaries of R. pulmo were mainly composed of water (93.7 ± 1.9% of wet weight), with organic matter (OM) and dry weight made respectively of proteins (761.76 ± 25.11 µ g mg&minus 1 and 45.7 ± 1.5%), lipids (192.17 ± 10.56 µ 1 and 9.6 ± 0.6%), and carbohydrates (59.66 ± 2.72 µ 1 and 3.7 ± 0.3%). The aqueous extract of R. pulmo gonads contained free amino acids, organic acids, and derivatives the lipid extract was composed of triglycerides (TG), polyunsaturated fatty acids (PUFAs), diunsaturated fatty acids (DUFAs), monounsaturated fatty acids (MUFAs), saturated fatty acids (SFAs), and minor components such as sterols and phospholipids. The R. pulmo oocyte lysate exhibited an antibacterial lysozyme-like activity (mean diameter of lysis of 9.33 ± 0.32 mm corresponding to 1.21 mg/mL of hen egg-white lysozyme). The occurrence of defense molecules is a crucial mechanism to grant healthy development of mature eggs and fertilized embryos (and the reproductive success of the species) by preventing marine bacterial overgrowth. As a corollary, these results call for future investigations for an exploitation of R. pulmo biomasses as a resource of bioactive metabolites of biotechnological importance including pharmaceuticals and nutrition. |
| Databáze: | OpenAIRE |
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