Polymerase Interactions with Wobble Mismatches in Synthetic Genetic Systems and Their Evolutionary Implications
| Autor: | Christian B. Winiger, Mariko F. Matsuura, Myong-Jung Kim, Steven A. Benner, Jennifer D. Moses, Shuichi Hoshika, Dietlind L. Gerloff, Ryan W. Shaw |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Speed wobble Base Pair Mismatch DNA polymerase Computational biology 010402 general chemistry 01 natural sciences Biochemistry Nucleobase Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Escherichia coli Base Pairing Polymerase Genetics biology Genetic systems DNA DNA Polymerase I 0104 chemical sciences 030104 developmental biology chemistry biology.protein DNA polymerase I Protein Binding |
| Zdroj: | Biochemistry |
| ISSN: | 1520-4995 0006-2960 |
| Popis: | In addition to completing the Watson-Crick nucleobase matching "concept" (big pairs with small, hydrogen bond donors pair with hydrogen bond acceptors), artificially expanded genetic information systems (AEGIS) also challenge DNA polymerases with a complete set of mismatches, including wobble mismatches. Here, we explore wobble mismatches with AEGIS with DNA polymerase 1 from Escherichia coli. Remarkably, we find that the polymerase tolerates an AEGIS:standard wobble that has the same geometry as the G:T wobble that polymerases have evolved to exclude but excludes a wobble geometry that polymerases have never encountered in natural history. These results suggest certain limits to "structural analogy" and "evolutionary guidance" as tools to help synthetic biologists expand DNA alphabets. |
| Databáze: | OpenAIRE |
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