Antioxidant and Antiproliferative Activities of Methanolic Extract from a Neglected Agricultural Product: Corn Cobs
| Autor: | Rony Lucas Silva Viana, Daisy Maria Machado, Vinícius Campelo Soeiro, Carmen Veríssima Ferreira, Raniere Fagundes Melo-Silveira, Rodrigo A. da Silva, Gabriel Pereira Fidelis, Leandro Silva da Costa, Hugo Alexandre Oliveira Rocha |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
apoptosis
HeLa cells maize phenolic compounds Antioxidant DPPH medicine.medical_treatment Pharmaceutical Science Apoptosis Analytical Chemistry HeLa chemistry.chemical_compound Superoxides Drug Discovery biology Superoxide Solid Phase Extraction Free Radical Scavengers Catalase Biochemistry Chemistry (miscellaneous) Molecular Medicine Oxidation-Reduction Cell Survival Trolox equivalent antioxidant capacity Zea mays Article Superoxide dismutase lcsh:QD241-441 Inhibitory Concentration 50 lcsh:Organic chemistry medicine Animals Humans Physical and Theoretical Chemistry Rats Wistar Cell Proliferation Plant Extracts Superoxide Dismutase Methanol Organic Chemistry Plant Components Aerial Ascorbic acid biology.organism_classification Antineoplastic Agents Phytogenic chemistry biology.protein Solvents Metallothionein Drug Screening Assays Antitumor |
| Zdroj: | Molecules Molecules, Vol 19, Iss 4, Pp 5360-5378 (2014) Molecules; Volume 19; Issue 4; Pages: 5360-5378 |
| ISSN: | 1420-3049 |
| Popis: | Neglected agricultural products (NAPs) are defined as discarded material in agricultural production. Corn cobs are a major waste of agriculture maize. Here, a methanolic extract from corn cobs (MEC) was obtained. MEC contains phenolic compounds, protein, carbohydrates (1.4:0.001:0.001). We evaluated the in vitro and in vivo antioxidant potential of MEC. Furthermore, its antiproliferative property against tumor cells was assessed through MTT assays and proteins related to apoptosis in tumor cells were examined by western blot. MEC showed no hydroxyl radical scavenger capacity, but it showed antioxidant activity in Total Antioxidant Capacity and DPPH scavenger ability assays. MEC showed higher Reducing Power than ascorbic acid and exhibited high Superoxide Scavenging activity. In tumor cell culture, MEC increased catalase, metallothionein and superoxide dismutase expression in accordance with the antioxidant tests. In vivo antioxidant test, MEC restored SOD and CAT, decreased malondialdehyde activities and showed high Trolox Equivalent Antioxidant Capacity in animals treated with CCl4. Furthermore, MEC decreased HeLa cells viability by apoptosis due an increase of Bax/Bcl-2 ratio, caspase 3 active. Protein kinase C expression increased was also detected in treated tumor cells. Thus, our findings pointed out the biotechnological potential of corn cobs as a source of molecules with pharmacological activity. |
| Databáze: | OpenAIRE |
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