Comparison of amniotic membrane versus the induced membrane for bone regeneration in long bone segmental defects using calcium phosphate cement loaded with BMP-2
| Autor: | Florelle Gindraux, Loïc Sentilhes, Nicolas L'Heureux, Marlène Durand, Robin Siadous, Mathilde Fenelon, Jean-Christophe Fricain, Agathe Grémare, Marion Etchebarne, Sylvain Catros |
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| Přispěvatelé: | Bioingénierie tissulaire (BIOTIS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux (UB) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Calcium Phosphates
Scaffold Materials science Bone Regeneration [SDV.BIO]Life Sciences [q-bio]/Biotechnology Long bone Bioengineering 02 engineering and technology Bone healing macromolecular substances 010402 general chemistry Bone morphogenetic protein 01 natural sciences Bone morphogenetic protein 2 Biomaterials Tissue engineering Osteogenesis medicine Animals Amnion Bone regeneration Decellularization Tissue Scaffolds technology industry and agriculture Bone Cements 021001 nanoscience & nanotechnology 0104 chemical sciences Rats medicine.anatomical_structure Mechanics of Materials 0210 nano-technology Biomedical engineering |
| Zdroj: | Materials Science and Engineering: C Materials Science and Engineering: C, 2021, 124, pp.112032. ⟨10.1016/j.msec.2021.112032⟩ |
| Popis: | Thanks to its biological properties, the human amniotic membrane (HAM) combined with a bone substitute could be a single-step surgical alternative to the two-step Masquelet induced membrane (IM) technique for regeneration of critical bone defects. However, no study has directly compared these two membranes. We first designed a 3D-printed scaffold using calcium phosphate cement (CPC). We assessed its suitability in vitro to support human bone marrow mesenchymal stromal cells (hBMSCs) attachment and osteodifferentiation. We then performed a rat femoral critical size defect to compare the two-step IM technique with a single-step approach using the HAM. Five conditions were compared. Group 1 was left empty. Group 2 received the CPC scaffold loaded with rh-BMP2 (CPC/BMP2). Group 3 and 4 received the CPC/BMP2 scaffold covered with lyophilized or decellularized/lyophilized HAM. Group 5 underwent a two- step induced membrane procedure with insertion of a polymethylmethacrylate (PMMA) spacer followed by, after 4 weeks, its replacement with the CPC/BMP2 scaffold wrapped in the IM. Micro-CT and histomorphometric analysis were performed after six weeks. Results showed that the CPC scaffold supported the proliferation and osteodifferentiation of hBMSCs in vitro. In vivo, the CPC/BMP2 scaffold very efficiently induced bone formation and led to satisfactory healing of the femoral defect, in a single-step, without autograft or the need for any membrane covering. In this study, there was no difference between the two-step induced membrane procedure and a single step approach. However, the results indicated that none of the tested membranes further enhanced bone healing compared to the CPC/BMP2 group. |
| Databáze: | OpenAIRE |
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