Decreased concentration of klotho and increased concentration of FGF23 in the cerebrospinal fluid of patients with narcolepsy
| Autor: | Fernando Morgadinho Santos Coelho, Sergio Tufik, Rosilene M. Elias, Denis Bernardi Bichuetti, Rosa M.A. Moysés, Gustavo Bruniera Peres Fernandes, Giuliano da Paz Oliveira |
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| Rok vydání: | 2021 |
| Předmět: |
Fibroblast growth factor 23
medicine.medical_specialty urologic and male genital diseases Pathogenesis 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Internal medicine medicine Humans Klotho Proteins Klotho Glucuronidase Narcolepsy Orexins business.industry Decreased Concentration Radioimmunoassay General Medicine medicine.disease Fibroblast Growth Factors Fibroblast Growth Factor-23 Endocrinology 030228 respiratory system Mann–Whitney U test business 030217 neurology & neurosurgery |
| Zdroj: | Sleep Medicine. 78:57-62 |
| ISSN: | 1389-9457 |
| DOI: | 10.1016/j.sleep.2020.11.037 |
| Popis: | Objective to explore the status of concentration of klotho and fibroblast growth factor 23 (FGF23) in cerebrospinal fluid (CSF) of patients with narcolepsy. Patients/methods 59 patients with narcolepsy and 17 control individuals were enrolled. We used radioimmunoassay, human klotho enzyme-linked immunosorbent assay (ELISA), human intact FGF23 ELISA and spectrophotometry to measure hypocretin-1, klotho, FGF-23 and phosphorus, respectively. T-Student Test was used to compare klotho and phosphate concentrations, Mann–Whitney U Test were used to compare FGF-23 levels between groups. ANOVA Test was used to compare klotho and phosphate CSF concentrations among narcolepsy patients with CSF hypocretin-1 110 pg/ml (HCRT+) versus control subjects. Results Klotho and phosphorus CSF levels were lower in narcoleptic patients than in control (908.18 ± 405.51 versus 1265.78 ± 523.26 pg/ml; p = 0.004 and 1.34 ± 0.25 versus 1.58 ± 0.23 mg/dl; p = 0.001, respectively). We found higher FGF-23 levels in narcoleptic patients (5.51 versus 4.00 pg/mL; p = 0.001). Klotho and phosphorus CSF levels were lower in both HCRT- and HCRT+ than controls. Moreover, there were higher FGF-23 levels in both HCRT-/HCRT+ groups versus controls. However, we did not find differences comparing HCRT- and HCRT+ groups, analyzing CSF klotho, FGF-23 or phosphorus levels. Conclusions Patients with narcolepsy have decreased CSF concentration of klotho and increased CSF levels of FGF-23. These findings may play a role in understanding the pathogenesis of narcolepsy. |
| Databáze: | OpenAIRE |
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