Regulation of breathing pattern by IL-10
| Autor: | John J. Greer, Ioannis Koutsourelakis, Vyronia Vassilakopoulou, Apostolos Papalois, Georgios A. Kastis, Adamantia Sotiriou, Fotis Perlikos, Eleftheria Mizi, Dimitrios Toumpanakis, Theodoros P. Vassilakopoulos, Georgios Prezerakos, Michael Yang, Maria Dettoraki, Charoula Eleni Giannakopoulou |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Physiology medicine.medical_treatment Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Breathing pattern Physiology (medical) Animals Medicine Mice Knockout Whole Body Plethysmography Medulla Oblongata business.industry Brain Carbon Dioxide Interleukin-10 Oxygen Interleukin 10 030104 developmental biology Cytokine Gene Expression Regulation Control of respiration Immunology Respiratory Physiological Phenomena business 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 317:R190-R202 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.00065.2019 |
| Popis: | Proinflammatory cytokines like interleukin-1β (IL-1β) affect the control of breathing. Our aim is to determine the effect of the anti-inflammatory cytokine IL-10 οn the control of breathing. IL-10 knockout mice (IL-10−/−, n = 10) and wild-type mice (IL-10+/+, n = 10) were exposed to the following test gases: hyperoxic hypercapnia 7% CO2-93% O2, normoxic hypercapnia 7% CO2-21% O2, hypoxic hypercapnia 7% CO2-10% O2, and hypoxic normocapnia 3% CO2-10% O2. The ventilatory function was assessed using whole body plethysmography. Recombinant mouse IL-10 (rIL-10; 10 μg/kg) was administered intraperitoneally to wild-type mice ( n = 10) 30 min before the onset of gas challenge. IL-10 was administered in neonatal medullary slices (10–30 ng/ml, n = 8). We found that IL-10−/−mice exhibited consistently increased frequency and reduced tidal volume compared with IL-10+/+mice during room air breathing and in all test gases (by 23.62 to 33.2%, P < 0.05 and −36.23 to −41.69%, P < 0.05, respectively). In all inspired gases, the minute ventilation of IL-10−/−mice was lower than IL-10+/+(by −15.67 to −22.74%, P < 0.05). The rapid shallow breathing index was higher in IL-10−/−mice compared with IL-10+/+mice in all inspired gases (by 50.25 to 57.5%, P < 0.05). The intraperitoneal injection of rIL-10 caused reduction of the respiratory rate and augmentation of the tidal volume in room air and also in all inspired gases (by −12.22 to −29.53 and 32.18 to 45.11%, P < 0.05, respectively). IL-10 administration in neonatal rat ( n = 8) in vitro rhythmically active medullary slice preparations did not affect either rhythmicity or peak amplitude of hypoglossal nerve discharge. In conclusion, IL-10 may induce a slower and deeper pattern of breathing. |
| Databáze: | OpenAIRE |
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