Additional file 1: Figure S1. of Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals

Autor: Livija Zlopasa, Brachner, Andreas, Foisner, Roland
Rok vydání: 2016
DOI: 10.6084/m9.figshare.c.3622076_d3
Popis: Catalytically dead Ankle1 mutant shuttles like wild-type Ankle1 but does not induce DNA damage response. (A) U2OS cells stably expressing GFP-Ankle1mut (GIY-YIG to GIY-AAA) were imaged using confocal microscopy without or after 3 h leptomycin B treatment. (B) U2OS cells were transiently transfected with Ankle1wt-V5 or Ankle1mut-V5 (GIY-YIG to GIY-AAA). Cells were analyzed by confocal immunofluorescence microscopy following staining with antibodies to V5 and γH2A.X. Scale bars: 10 μm. (PDF 990 kb)
Databáze: OpenAIRE