In vitro regeneration of wild chervil (Anthriscus sylvestris L.)
| Autor: | Herman J. Woerdenbag, Oktavia Hendrawati, Jacques Hille, Oliver Kayser, Wim J. Quax |
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| Přispěvatelé: | Pharmaceutical Technology and Biopharmacy, Groningen Biomolecular Sciences and Biotechnology, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Groningen Research Institute of Pharmacy |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Anthriscus sylvestris
Somatic embryogenesis STRATEGIES Deoxypodophyllotoxin Organogenesis AROMATIC PLANTS Plant Science PHENOTYPE Hypocotyl AGROBACTERIUM-RHIZOGENES BELGIAN-ENDIVE SOMATIC EMBRYOGENESIS SUSPENSION CULTURES Botany medicine Regeneration Plant Tissue Culture biology fungi food and beverages Micropropagation biology.organism_classification Tissue culture Podophyllotoxin Callus Shoot CELLS TUMEFACIENS Biotechnology Explant culture medicine.drug GENETIC-TRANSFORMATION |
| Zdroj: | In Vitro Cellular & Developmental Biology In vitro cellular & developmental biology-Plant, 48(3), 355-361 |
| ISSN: | 1054-5476 |
| Popis: | Anthriscus sylvestris (L.) Hoffm. (Apiaceae) is a common wild plant that accumulates the lignan deoxypodophyllotoxin. Deoxypodophyllotoxin can be hydroxylated at the C-7 position in recombinant organisms yielding podophyllotoxin, which is used as a semi-synthetic precursor for the anticancer drugs, etoposide phosphate and teniposide. As in vitro regeneration of A. sylvestris has not yet been reported, development of a regeneration protocol for A. sylvestris would be useful as a micropropagation tool and for metabolic engineering of the plant. Calli were induced from hypocotyl explants and transferred to shoot induction medium containing zeatin riboside. Regenerated shoots were obtained within 6 mo and were transferred onto growth regulator-free root induction medium containing 1% sucrose. Regenerated plants transferred to soil and acclimatized in a greenhouse. Plants were transferred to the field with a 100% survival rate. Regenerated plants flowered and were fully fertile. This is the first report of complete regeneration of A. sylvestris via shoot organogenesis from callus. |
| Databáze: | OpenAIRE |
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