Coagulase-negative staphylococci species affect biofilm formation of other coagulase-negative and coagulase-positive staphylococci
| Autor: | François Malouin, Mario Jacques, Andréanne Blondeau, Josée Labrie, Daphnée Lamarche, Coralie Goetz, Annie M. Gaudreau, Yannick D. N. Tremblay |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Coagulase
0301 basic medicine Staphylococcus aureus Staphylococcus 030106 microbiology Staphylococcus chromogenes medicine.disease_cause Microbiology Agar plate 03 medical and health sciences Staphylococcus simulans Genetics medicine Animals Humans Mastitis Bovine biology Biofilm Staphylococcal Infections biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Phenotype Mastitis 030104 developmental biology Biofilms Cattle Female Animal Science and Zoology Food Science |
| Zdroj: | Journal of Dairy Science. 100:6454-6464 |
| ISSN: | 0022-0302 |
| Popis: | Coagulase-negative staphylococci (CNS) are considered to be commensal bacteria in humans and animals, but are now also recognized as etiological agents in several infections, including bovine mastitis. Biofilm formation appears to be an important factor in CNS pathogenicity. Furthermore, some researchers have proposed that CNS colonization of the intramammary environment has a protective effect against other pathogens. The mechanisms behind the protective effect of CNS have yet to be characterized. The aim of this study was to evaluate the effect of CNS isolates with a weak-biofilm phenotype on the biofilm formation of other staphylococcal isolates. We selected 10 CNS with a weak-biofilm phenotype and 30 staphylococcal isolates with a strong-biofilm phenotype for this study. We measured biofilm production by individual isolates using a standard polystyrene microtiter plate assay and compared the findings with biofilm produced in mixed cultures. We confirmed the results using confocal microscopy and a microfluidic system with low shear force. Four of the CNS isolates with a weak-biofilm phenotype (Staphylococcus chromogenes C and E and Staphylococcus simulans F and H) significantly reduced biofilm formation in approximately 80% of the staphylococcal species tested, including coagulase-positive Staphylococcus aureus. The 4 Staph. chromogenes and Staph. simulans isolates were also able to disperse pre-established biofilms, but to a lesser extent. We also performed a deferred antagonism assay and recorded the number of colony-forming units in the mixed-biofilm assays on differential or selective agar plates. Overall, CNS with a weak-biofilm phenotype did not inhibit the growth of isolates with a strong-biofilm phenotype. These results suggest that some CNS isolates can negatively affect the ability of other staphylococcal isolates and species to form biofilms via a mechanism that does not involve growth inhibition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|