Polymerase chain reaction based cDNA cloning of wheat profilin: a potential plant allergen
| Autor: | P. Rozynek, Xaver Baur, B. Welticke, K. May-Taube, Hans-Peter Rihs |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Immunology Molecular Sequence Data macromolecular substances medicine.disease_cause Polymerase Chain Reaction Homology (biology) law.invention Profilins Allergen Contractile Proteins law Complementary DNA medicine Immunology and Allergy Amino Acid Sequence Cloning Molecular Peptide sequence Polymerase chain reaction Triticum Plant Proteins biology Base Sequence Sequence Homology Amino Acid Microfilament Proteins Nucleic acid sequence food and beverages General Medicine Allergens Molecular biology Subcloning Profilin biology.protein |
| Zdroj: | International archives of allergy and immunology. 105(2) |
| ISSN: | 1018-2438 |
| Popis: | Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high amino acid sequence identity with the birch pollen allergen Bet v II suggests that wheat profilin may be a potential plant allergen, relevant in food allergy. |
| Databáze: | OpenAIRE |
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