Occurrence of histamine-production-increasing factor in the postanaphylactic phase of allergic inflammation
| Autor: | Masako Watanabe, Kazuo Ohuchi, Susumu Tsurufuji, Kimiko Kawarasaki, Noriyasu Hirasawa |
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| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty Hot Temperature Time Factors Immunology Inflammation Bone Marrow Cells Histidine Decarboxylase Histamine Release Allergic inflammation chemistry.chemical_compound Bone Marrow Internal medicine Immunology and Allergy Medicine Animals Trypsin Anaphylaxis Histamine Production business.industry Rats Inbred Strains General Medicine Methylhistidines Histidine decarboxylase Rats Molecular Weight Endocrinology chemistry Histidine decarboxylase activity Liberation Interleukin-3 medicine.symptom Pouch business Histamine |
| Zdroj: | International archives of allergy and applied immunology. 88(4) |
| ISSN: | 0020-5915 |
| Popis: | Experimental study on histamine liberation in a postanaphylactic phase of allergy was carried out employing an air pouch model of allergic inflammation in rats. The antigen used was azobenzenearsonate-conjugated acetyl bovine serum albumin. Synthesis and liberation of histamine took place in the inflammatory pouch in the dorsum of the allergic rats, and brought about a gradual rise in the histamine level in the pouch fluid with a peak at 24 h after the antigen challenge. The time course of the histamine level in the pouch fluid was quite similar to that of histidine decarboxylase activities in the inflammatory tissues. α-Fluoromethylhistidine reduced the histamine level in the postanaphylactic phase, although it had been ineffective in the anaphylactic phase. A substance capable of increasing histamine production by bone marrow cells was found in the pouch fluid of allergic rats, while it was absent in the normal rat serum and pouch fluid of nonsensitized rats. The histamine-production-increasing activity rose until 24 h after the antigen challenge, but fell at 48 h in parallel with changes both of histamine levels in the pouch fluid and histidine decarboxylase activity in inflammatory tissues. The histamine-production-increasing factor is thought to be a protein, since it was inactivated by treatment with heat (70 °C for 30 min) or trypsin. Its molecular weight was estimated to be between 25,000 and 40,000. |
| Databáze: | OpenAIRE |
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