Differential induction and intracellular localization of SCG10 messenger RNA is associated with neuronal differentiation
| Autor: | J. W. Sentry, Ron P. Weinberger, Anthony J. Hannan, Robert C. Henke, Peter L. Jeffrey |
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| Rok vydání: | 1996 |
| Předmět: |
Neurite
Cellular differentiation Molecular Sequence Data Superior Cervical Ganglion In situ hybridization Biology Nervous System Rats Sprague-Dawley Cerebellum Gene expression Neurites Animals Nervous System Physiological Phenomena Amino Acid Sequence Nerve Growth Factors RNA Messenger Promoter Regions Genetic Cells Cultured In Situ Hybridization Neurons Regulation of gene expression Messenger RNA Base Sequence General Neuroscience Gene Expression Regulation Developmental Membrane Proteins RNA Cell Differentiation Translation (biology) Sequence Analysis DNA Blotting Northern Molecular biology Rats Microtubule Proteins Carrier Proteins |
| Zdroj: | Neuroscience. 72:889-900 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(95)00593-5 |
| Popis: | The differentiation of neurons involves the establishment of distinct molecular compartments which regulate neuronal shape and function. This requires targeting of specific gene products to growth-associated regions of the neuron. We have investigated the temporal and spatial regulation of SCG10 gene expression during neuronal differentiation. There are two SCGIO messenger RNAs, 1 and 2 kb in length, which encode the same growth-associated protein. These messenger RNAs were found to be differentially regulated during the onset of neurite outgrowth in early rat cerebellum development. In PC12 cells, the two SCG10 messenger RNAs were shown to be differentially induced by nerve growth factor. Regulation of the 2 kb messenger RNA, but not the 1 kb messenger RNA, is dependent on the differentiation of PC 12 cells, indicating post-transcriptional regulation of SCG10 expression during neurite outgrowth. Spatial regulation of the 2 kb SCG10 messenger RNA distribution during brain development was examined byin situ hybridization. The 2 kb messenger RNA was found to be localized to the neuronal pole where neurite outgrowth was occurring, within differentiating neuronsin vivo. Intracellular localization of SCG10 messenger RNA was also observed in differentiating primary cultured neurons, with the 2 kb messenger RNA transported into growing neurites during the development of neuronal polarity. In neurons which had developed polarity, the 2 kb SCG10 messenger RNA was consistently found in the cell body and axon. This study demonstrates both temporal and spatial post-transcriptional regulation of SCG10 expression which is associated with neurite outgrowth. The directed transport and positional translation of SCG10 messenger RNA provide a potential mechanism for protein targeting and the creation of molecular compartments during neuronal differentiation. |
| Databáze: | OpenAIRE |
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