Pathologic Changes and Glucose Homeostasis According to Expression of Human Islet Amyloid Polypeptide in Type 2 Diabetic Patients
| Autor: | Seung Hoon Oh, Kee Taek Jang, Ji Young Park, Sook Young Kim, Yong Ki Min, Jae Hoon Chung, Hee Sung No, Moon Kyu Lee, Jin Seok Heo, Dong Wook Choi, Sun Wook Kim, You Ran Ahn, Young-Seok Kim, Jae Hyeon Kim, Seong Ho Choi, Kwang-Won Kim, Myung-Shik Lee |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Blood Glucose Male Amyloid endocrine system medicine.medical_specialty Histology Gene Expression Biology Glucagon Article Islets of Langerhans Internal medicine Diabetes mellitus medicine Humans Glucose homeostasis Pancreas Aged Cell Proliferation Glucose tolerance test geography geography.geographical_feature_category medicine.diagnostic_test Glucose Tolerance Test Middle Aged Islet medicine.disease Islet Amyloid Polypeptide medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 2 Apoptosis Female Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 58:731-740 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/jhc.2010.955906 |
| Popis: | The amount of amyloid detectable in islets varies, and is not always correlated with degree of β-cell loss. It has been hypothesized that islet amyloid polypeptide (IAPP) aggregation causes β-cell dysfunction. In this study, we investigated the relationship between IAPP expression and glucose homeostasis in pancreatectomized patients. Human pancreatic head tissues were collected from 46 pancreatic tumor patients. We divided the diabetic cases into two groups, patients with higher IAPP–expressing islets (DM-H) and patients with lower IAPP–expressing islets (DM-L), and compared both groups to patients with normal glucose tolerance (NGT). Interestingly, oral glucose tolerance test analyses showed that DM-L patients had significantly higher glucose levels and lower C-peptide levels than DM-H patients. Furthermore, the DM-H group showed a relative β-cell volume similar to that of the NGT group, as well as a significantly higher relative β-cell volume than the DM-L group. The DM-L group was significantly higher than the DM-H group, not only in the rates of replication and apoptosis, but also in the nuclear C/EBP homologous protein and the ratio of oligomer to IAPP. Thus, IAPP expression may not be an indicator of cell death induction. IAPP, including oligomer, may be an important determinant in the fate of islet β-cells. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 58:731–740, 2010) |
| Databáze: | OpenAIRE |
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