Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination
| Autor: | Yuki Takada-Horisawa, Kaho Okamura, Kimi Araki, Naoki Tani, Michihiko Sugimoto, Kei-ichiro Ishiguro, Kazumasa Takemoto, Sayoko Fujimura, Mariko Yamane, Nobuhiro Tanno |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male DNA repair genetic processes RAD51 Cell Cycle Proteins Chromosome DSB General Biochemistry Genetics and Molecular Biology Chromosome segregation 03 medical and health sciences Mice 0302 clinical medicine Meiosis Spermatocytes Recombinase Animals DNA Breaks Double-Stranded Homologous Recombination Spermatogenesis lcsh:QH301-705.5 meiotic recombination Chemistry fungi Phosphate-Binding Proteins Chiasma Cell biology enzymes and coenzymes (carbohydrates) 030104 developmental biology lcsh:Biology (General) health occupations DMC1 Rad51 Recombinase Homologous recombination 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 31, Iss 8, Pp-(2020) |
| ISSN: | 2211-1247 |
| Popis: | Summary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. Meiotic recombination is initiated by DNA double-strand break (DSB) followed by multiple processes of DNA repair. The exact mechanisms for how recombinases localize to DSB remain elusive. Here, we show that C19orf57/4930432K21Rik/BRME1 is a player for meiotic recombination in mice. C19orf57/4930432K21Rik/BRME1 associates with single-stranded DNA (ssDNA) binding proteins, BRCA2 and MEILB2/HSF2BP, which are critical recruiters of recombinases onto DSB sites. Disruption of C19orf57/4930432K21Rik/BRME1 shows severe impact on DSB repair and male fertility. Remarkably, removal of ssDNA binding proteins from DSB sites is delayed, and reciprocally, the loading of RAD51 and DMC1 onto resected ssDNA is impaired in Brme1 knockout (KO) spermatocytes. We propose that C19orf57/4930432K21Rik/BRME1 modulates localization of recombinases to meiotic DSB sites through the interaction with the BRCA2-MEILB2/HSF2BP complex during meiotic recombination. |
| Databáze: | OpenAIRE |
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