Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain

Autor: Matthew J. Moscou, Aleksandra Białas, Clare E. M. Stevenson, Mark J. Banfield, Thorsten Langner, Mauricio P Contreras, David M. Lawson, Jan Sklenar, Ryohei Terauchi, Ronny Kellner, Adeline Harant, Sophien Kamoun
Rok vydání: 2021
Předmět:
Models
Molecular
0106 biological sciences
0301 basic medicine
Plant Biology
01 natural sciences
Sequence Analysis
Protein
Convergent evolution
Receptors
Immunologic
Biology (General)
Phylogeny
Plant Proteins
0303 health sciences
Phylogenetic tree
Effector
General Neuroscience
General Medicine
Phenotype
Host-Pathogen Interactions
Medicine
Research Article
Genotype
Architecture domain
QH301-705.5
Science
Computational biology
Biology
Genes
Plant
Poaceae
General Biochemistry
Genetics and Molecular Biology
NLR
Domain (software engineering)
03 medical and health sciences
Protein Domains
plant defense
Metals
Heavy
evolution
Alleles
Plant Diseases
030304 developmental biology
General Immunology and Microbiology
rice
fungi
Fungi
Oryza
HMA domain
030104 developmental biology
Other
Adaptation
Sequence Alignment
010606 plant biology & botany
Zdroj: eLife, Vol 10 (2021)
eLife
ISSN: 2050-084X
Popis: A subset of plant NLR immune receptors carry unconventional integrated domains in addition to their canonical domain architecture. One example is rice Pik-1 that comprises an integrated heavy metal–associated (HMA) domain. Here, we reconstructed the evolutionary history of Pik-1 and its NLR partner, Pik-2, and tested hypotheses about adaptive evolution of the HMA domain. Phylogenetic analyses revealed that the HMA domain integrated into Pik-1 before Oryzinae speciation over 15 million years ago and has been under diversifying selection. Ancestral sequence reconstruction coupled with functional studies showed that two Pik-1 allelic variants independently evolved from a weakly binding ancestral state to high-affinity binding of the blast fungus effector AVR-PikD. We conclude that for most of its evolutionary history the Pik-1 HMA domain did not sense AVR-PikD, and that different Pik-1 receptors have recently evolved through distinct biochemical paths to produce similar phenotypic outcomes. These findings highlight the dynamic nature of the evolutionary mechanisms underpinning NLR adaptation to plant pathogens.
Databáze: OpenAIRE
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