Expression of N-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines
| Autor: | Nevis Fregien, Michael Pierce, Irene S. Margitich, Guang Shing Perng, Mohamed G. Shoreibah |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Molecular Sequence Data Mutant N-Acetylglucosaminyltransferases Polymerase Chain Reaction Biochemistry Gene Expression Regulation Enzymologic Cell Line Mice Gene expression Glycosyltransferase medicine Animals Humans Tissue Distribution RNA Messenger DNA Primers Messenger RNA Base Sequence biology Skeletal muscle medicine.disease Molecular biology Rats Lymphoma medicine.anatomical_structure Cell culture biology.protein Female A431 cells |
| Zdroj: | Glycobiology. 4:867-871 |
| ISSN: | 1460-2423 0959-6658 |
| DOI: | 10.1093/glycob/4.6.867 |
| Popis: | The expression of N-acetylglucosaminyltransferase V (Glc-NAc-T V) is increased in many oncogenically transformed cells and in cell lines which are induced to proliferate. In order to characterize the regulation of GlcNAc-T V at the molecular level, we have examined the expression of Glc-NAc-T V mRNAs in a number of mouse tissues and in several human and rodent cell lines. The GlcNAc-T V mRNA is expressed in different amounts in the various mouse tissues, with the greatest amount observed in brain, followed by thymus, kidney, lung, intestine, heart and stomach, and no transcripts detected in liver or skeletal muscle. Measurements of GlcNAc-T V enzymatic activity, by contrast, show brain to have lower levels of activity than several of the other tissues, suggesting possible post-translational regulation. We find that there are two GlcNAc-T V transcripts in most of the RNAs analysed. The rodent cell lines all express both a 7.5 and a 9.5 kb mRNA, with the smaller transcript being more abundant. The human cells have mRNAs of 4.5 and 9.5 kb. Both mRNAs are expressed in HepG2 and MCF-7 cells, while A431 cells express only the 4.5 kb mRNA and HL60 cells express only the 9.5 kb transcript. Furthermore, only the 9.5 kb mRNA appears to be increased, along with activity, when HepG2 cells are stimulated to proliferate, suggesting that the two mRNAs may be under different regulatory controls. Also, a GlcNAc-T V-deficient, mutant lymphoma cell line, PHAR2.1, was found to express mRNAs which are larger than the normal mRNAs, possible due to an insertion or aberrant splicing, resulting in a defective mRNA. |
| Databáze: | OpenAIRE |
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