Analyses of apoptosis and DNA damage in bovine cumulus cells after in vitro maturation with different copper concentrations: consequences on early embryo development
| Autor: | Guillermo Alberto Mattioli, Juan Patricio Anchordoquy, Matias Angel Sirini, Diana Esther Rosa, Cecilia Cristina Furnus, Juan Alberto Testa, Juan Mateo Anchordoquy |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male DNA damage medicine.medical_treatment In Vitro Oocyte Maturation Techniques EARLY DEVELOPMENT Apoptosis Early development Fertilization in Vitro Biology DNA DAMAGE MINERALS Ciencias Biológicas 03 medical and health sciences medicine Animals Blastocyst Cells Cultured Minerals In vitro fertilisation Cumulus Cells Dose-Response Relationship Drug urogenital system Ciencias Veterinarias Embryogenesis Cell Biology Oocyte Molecular biology In vitro maturation APOPTOSIS 030104 developmental biology medicine.anatomical_structure embryonic structures Oocytes Cattle Female Biología Reproductiva CIENCIAS NATURALES Y EXACTAS Copper Developmental Biology DNA Damage |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 1469-8730 |
| Popis: | The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus–oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu. Cumulus expansion and viability of CC did not show differences in COC treated with 0, 20, 40, or 60 µg/dl Cu during IVM. After in vitro fertilization (IVF), cleavage rates were higher in COC and DO + CC (denuded oocytes + CC) with or without Cu than in DO. Independently of CC presence (COC, DO + CC or DO) the blastocyst rates were higher when 60 µg/dl Cu was added to IVM medium compared to medium alone. These results indicate that Cu supplementation to IVM medium: (i) decreased DNA damage and apoptosis in CC; (ii) did not modify oocyte viability and cumulus expansion; and (iii) improved subsequent embryo development up to blastocyst stage regardless of CC presence during IVM. Instituto de Genética Veterinaria Facultad de Ciencias Veterinarias Facultad de Ciencias Médicas |
| Databáze: | OpenAIRE |
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