Prevalence of ESBL-producing Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods

Autor: Milena Ćmiel, Patrycja Róg, Danuta Dzierzanowska, Alicja Słoczyńska, Renata Wolinowska, Stefan Tyski, Agnieszka E. Laudy, Katarzyna Smolińska-Król, Jan Patzer, Bohdan Starościak
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Imipenem
lcsh:Medicine
medicine.disease_cause
Pathology and Laboratory Medicine
Geographical Locations
Database and Informatics Methods
Disk Diffusion Antimicrobial Tests
Antibiotics
Genotype
Prevalence
Medicine and Health Sciences
polycyclic compounds
lcsh:Science
Cross Infection
Multidisciplinary
biology
Antimicrobials
Pseudomonas Aeruginosa
Drugs
Sulbactam
Enterobacteriaceae
Electrophoresis
Gel
Pulsed-Field
Bacterial Pathogens
Europe
Synergy Testing
Medical Microbiology
Pathogens
Sequence Analysis
medicine.drug
Research Article
Bioinformatics
030106 microbiology
Bacterial Disk Diffusion
Nucleotide Sequencing
Research and Analysis Methods
Microbiology
beta-Lactam Resistance
beta-Lactamases
Antibiotic Susceptibility Testing
03 medical and health sciences
Amino Acid Sequence Analysis
Clavulanic acid
Pseudomonas
Microbial Control
Multiplex polymerase chain reaction
Pulsed-field gel electrophoresis
medicine
Humans
Pseudomonas Infections
Molecular Biology Techniques
Sequencing Techniques
Microbial Pathogens
Molecular Biology
Pharmacology
Bacteria
Pseudomonas aeruginosa
lcsh:R
Organisms
Biology and Life Sciences
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
bacterial infections and mycoses
Pharmacologic Analysis
People and Places
bacteria
lcsh:Q
Poland
Multiplex Polymerase Chain Reaction
Zdroj: PLoS ONE, Vol 12, Iss 6, p e0180121 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Knowledge of the prevalence of ESBL enzymes among P. aeruginosa strains compared to the Enterobacteraiceae family is limited. The phenotypic tests recommended by EUCAST for the detection of ESBL-producing Enterobacteriaceae are not always suited for P. aeruginosa strains. This is mainly due to the presence of other families of ESBLs in P. aeruginosa isolates more often than in Enterobacteriaceae, production of natural AmpC cephalosporinase and its overexpression, and co-production of metallo-β-lactamases. The aim of this study was to determine the occurrence of ESBLs in P. aeruginosa isolated from patients from hospitals in Warsaw, to evaluate the ESBL production of these isolates using currently available phenotypic tests, their modifications, multiplex PCR and molecular typing of ESBL-positive isolates by PFGE. Clinical isolates of P. aeruginosa were collected in 2000-2014 from four Warsaw hospitals. Based on the data obtained in this study, we suggest using three DDST methods with inhibitors, such as clavulanic acid, sulbactam and imipenem, to detect ESBL-producing P. aeruginosa strains. Depending on the appearance of the plates, we suggest a reduction in the distance between discs with antibiotics to 15 mm and the addition of boronic acid at 0.4 mg per disc. The analysed isolates carried genes encoding ESBL from the families VEB (69 isolates with VEB-9), GES (6 with GES-1, 1 GES-5, 5 GES-13 and 2 with GES-15), OXA-2 (12 with OXA-15, 1 OXA-141, 1 OXA-210, 1 OXA-543 and 1 with OXA-544) and OXA-10 (5 isolates with OXA-74 and one with OXA-142). The most important result of this study was the discovery of three new genes, blaGES-15, blaOXA-141 and blaOXA-142; their nucleotide sequences have been submitted to the NCBI GenBank. It is also very important to note that this is the first report on the epidemiological problem of VEB-9-producing bacterial strains, not only in Poland but also worldwide.
Databáze: OpenAIRE
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