Novel chromosomal insertions of ISEcp1-bla CTX-M-15 and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli
| Autor: | Atmika Paudel, Tuvshinzaya Zorigt, Hideaki Higashi, Bernard M. Hang’ombe, Christone Kaile, Misheck Shawa, Manyando Simbotwe, Yoshikazu Furuta, Gillan Mulenga, Evans Mulenga, Maron Mubanga |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) bla CTX-M Klebsiella pneumoniae 030106 microbiology Zambia Chromosomal insertion Infectious and parasitic diseases RC109-216 Genome 03 medical and health sciences Plasmid Chromosomal Insertion Enterobacter cloacae Escherichia coli polycyclic compounds Medicine Pharmacology (medical) AMR bla(CTX-M) Gene Genetics biology business.industry Extended spectrum beta-lactamase Public Health Environmental and Occupational Health ISEcp1 biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Enterobacteriaceae Multiple drug resistance 030104 developmental biology Infectious Diseases Extended spectrum β-lactamase business |
| Zdroj: | Antimicrobial Resistance and Infection Control, Vol 10, Iss 1, Pp 1-16 (2021) |
| ISSN: | 2047-2994 |
| Popis: | Background The epidemiology of extended-spectrum β-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. blaCTX-M genes, encoding CTX-M-type ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that blaCTX-M-harboring strains often exhibit multidrug resistance (MDR), it is important to investigate the association between chromosomally integrated blaCTX-M and the presence of additional antimicrobial resistance (AMR) genes, and to identify other relevant genetic elements. Methods A total of 46 clinical isolates of cefotaxime-resistant Enterobacteriaceae (1 Enterobacter cloacae, 9 Klebsiella pneumoniae, and 36 Escherichia coli) from Zambia were subjected to whole-genome sequencing (WGS) using MiSeq and MinION. By reconstructing nearly complete genomes, blaCTX-M genes were categorized as either chromosomal or plasmid-borne. Results WGS-based genotyping identified 58 AMR genes, including four blaCTX-M alleles (i.e., blaCTX-M-14, blaCTX-M-15, blaCTX-M-27, and blaCTX-M-55). Hierarchical clustering using selected phenotypic and genotypic characteristics suggested clonal dissemination of blaCTX-M genes. Out of 45 blaCTX-M gene-carrying strains, 7 harbored the gene in their chromosome. In one E. cloacae and three E. coli strains, chromosomal blaCTX-M-15 was located on insertions longer than 10 kb. These insertions were bounded by ISEcp1 at one end, exhibited a high degree of nucleotide sequence homology with previously reported plasmids, and carried multiple AMR genes that corresponded with phenotypic AMR profiles. Conclusion Our study revealed the co-occurrence of ISEcp1-blaCTX-M-15 and multiple AMR genes on chromosomal insertions in E. cloacae and E. coli, suggesting that ISEcp1 may be responsible for the transposition of diverse AMR genes from plasmids to chromosomes. Stable retention of such insertions in chromosomes may facilitate the successful propagation of MDR clones among these Enterobacteriaceae species. |
| Databáze: | OpenAIRE |
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