Analytical ultracentrifuge: an ideal tool for characterization of non-coding RNAs
| Autor: | Trushar R. Patel, Darren L. Gemmill, M. Quadir Siddiqui, Maulik D. Badmalia, Tyler Mrozowich |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification 030103 biophysics RNA Untranslated Biomolecule Biophysics RNA HOTAIR General Medicine Computational biology Characterization (materials science) 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry RNA Viral XIST Ultracentrifuge Ultracentrifugation Large size DNA |
| Zdroj: | European Biophysics Journal. 49:809-818 |
| ISSN: | 1432-1017 0175-7571 |
| DOI: | 10.1007/s00249-020-01470-9 |
| Popis: | Analytical ultracentrifugation (AUC) has emerged as a robust and reliable technique for biomolecular characterization with extraordinary sensitivity. AUC is widely used to study purity, conformational changes, biomolecular interactions, and stoichiometry. Furthermore, AUC is used to determine the molecular weight of biomolecules such as proteins, carbohydrates, and DNA and RNA. Due to the multifaceted role(s) of non-coding RNAs from viruses, prokaryotes, and eukaryotes, research aimed at understanding the structure-function relationships of non-coding RNAs is rapidly increasing. However, due to their large size, flexibility, complicated secondary structures, and conformations, structural studies of non-coding RNAs are challenging. In this review, we are summarizing the application of AUC to evaluate the homogeneity, interactions, and conformational changes of non-coding RNAs from adenovirus as well as from Murray Valley, Powassan, and West Nile viruses. We also discuss the application of AUC to characterize eukaryotic long non-coding RNAs, Xist, and HOTAIR. These examples highlight the significant role AUC can play in facilitating the structural determination of non-coding RNAs and their complexes. |
| Databáze: | OpenAIRE |
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