12-Lipoxygenase in Opioid-Induced Delayed Cardioprotection

Autor: Richard A. Bundey, Anna K. Hsu, Ryan M. Fryer, Leonard O.V. Eusebi, Eric R. Gross, Kasem Nithipatikom, Garrett J. Gross, Steven R. Gullans, Marilyn A. Isbell, Hemal H. Patel, Roderick V. Jensen, Paul A. Insel
Rok vydání: 2003
Předmět:
Male
Agonist
Spectrometry
Mass
Electrospray Ionization
medicine.medical_specialty
Cardiotonic Agents
Physiology
medicine.drug_class
Metabolite
Blotting
Western
Myocardial Reperfusion Injury
Phenidone
Arachidonate 12-Lipoxygenase
Piperazines
Rats
Sprague-Dawley
chemistry.chemical_compound
Lipoxygenase
Receptors
Opioid
delta
Internal medicine
medicine
Animals
12-Hydroxy-5
8
10
14-eicosatetraenoic Acid
Lipoxygenase Inhibitors
RNA
Messenger
Enzyme Inhibitors
Opioid peptide
Oligonucleotide Array Sequence Analysis
Cardioprotection
Arachidonic Acid
biology
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Gene Expression Profiling
Myocardium
Hemodynamics
Rats
Baicalein
Kinetics
Endocrinology
Benzamides
Ischemic Preconditioning
Myocardial
biology.protein
Ischemic preconditioning
Cardiology and Cardiovascular Medicine
Zdroj: Circulation Research. 92:676-682
ISSN: 1524-4571
0009-7330
Popis: 12-Lipoxygenase (12-LO) has been shown to be a factor in acute ischemic preconditioning (IPC) in the isolated rat heart; however, no studies have been reported in delayed PC. We characterized the role of 12-LO in an intact rat model of delayed PC induced by a δ-opioid agonist SNC-121 (SNC). Rats were pretreated with SNC and allowed to recover for 24 hours. They were then treated with either baicalein or phenidone, 2 selective 12-LO inhibitors. In addition, SNC-pretreated rats had plasma samples isolated at different times after ischemia-reperfusion for liquid chromatographic–mass spectrometric analysis of the major metabolic product of 12-LO, 12-HETE. Similar studies were conducted with inhibitors. Gene array data showed a significant induction of 12-LO message ( P P
Databáze: OpenAIRE
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