Molecular cloning and characterization of a novel stem-specific gene from Camptotheca acuminata
| Autor: | Yan Pi, You Rong Chai, Xiao Fen Sun, Zhihua Liao, Kexuan Tang, Yi Fu Gong, Peng Wang, Yongzhen Pang, Hai Nian Zeng |
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| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Protein Conformation Molecular Sequence Data Molecular cloning Biology Biochemistry Homology (biology) Cytochrome P-450 Enzyme System Complementary DNA Camptotheca Amino Acid Sequence Cloning Molecular Molecular Biology Gene Southern blot Plant Proteins Genetics Plants Medicinal Phenylpropanoid Base Sequence Sequence Homology Amino Acid food and beverages General Medicine Monooxygenase Molecular biology Open reading frame Plant Structures Sequence Alignment |
| Zdroj: | Journal of biochemistry and molecular biology. 39(1) |
| ISSN: | 1225-8687 |
| Popis: | In higher plants, P450s participate in the biosynthesis of many important secondary metabolites. Here we reported for the first time the isolation of a new cytochrome P450 cDNA that expressed in a stem-specific manner from Camptotheca acuminata (designated as CaSS), a native medicinal plant species in China, using RACE-PCR. The full-length cDNA of CaSS was 1735 bp long containing a 1530 bp open reading frame (ORF) encoding a polypeptide of 509 amino acids. Bioinformatic analysis revealed that CASS contained a heme-binding domain PFGXGRRXCX and showed homology to other plant cytochrome P450 monooxygenases and hydroxylases. Southern blotting analysis revealed that there was only one copy of the CaSS present in the genome of Camptotheca acuminata. Northern blotting analysis revealed that CaSS expressed, in a tissue-specific manner, highly in stem and lowly in root, leaf and flower. Our study suggests that CaSS is likely to be involved in the phenylpropanoid pathway. |
| Databáze: | OpenAIRE |
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