Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme

Autor: Nuria Tormo, María López Díaz, Jesús Rodríguez-Baño, Luis Anibarro, Lucía Blasco, Maria Tomas, Celia Mayer, Álvaro Pascual Hernández, Simona Mihaela Iftimie, Jordi Vila, German Bou, Ana Otero, Francisco Javier Casas-Ciria, Jerónimo Pachón, Andrea Muras, María de los Angeles Pallarés González, María Pilar Alonso García, Juan Pablo Horcajada, Laura Fernandez-Garcia, María de la Luz Cádiz-Gurrea, José Miguel Cisneros, Federico M. Ruiz, Azucena Rodriguez-Guardado
Přispěvatelé: Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Instituto de Salud Carlos III, European Commission
Rok vydání: 2017
Předmět:
0301 basic medicine
Acinetobacter baumannii
Hydrolases
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
4-Butyrolactone
Microbial Physiology
Medicine and Health Sciences
lcsh:Science
Pathogen
AidA
Cross Infection
Multidisciplinary
biology
Acinetobacter
Quorum Sensing
Clinical strains
Bacterial Pathogens
Enzymes
Quenching enzyme
Quorum Quenching
Medical Microbiology
Hyperexpression Techniques
Pathogens
A. baumannii
Research Article
Pathogen Motility
Virulence Factors
030106 microbiology
Down-Regulation
Research and Analysis Methods
Microbiology
Bacterial genetics
03 medical and health sciences
Extraction techniques
Bacterial Proteins
Intensive care
Escherichia coli
Homoserine
Gene Expression and Vector Techniques
Molecular Biology Techniques
Gene
Microbial Pathogens
Molecular Biology
Molecular Biology Assays and Analysis Techniques
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Bacteriology
Hydrolase Gene
biology.organism_classification
RNA extraction
Quorum sensing
Genes
Bacterial
Biofilms
Enzymology
lcsh:Q
Bacterial Biofilms
Quorum
Zdroj: Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname
Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela
Universidad de Santiago de Compostela (USC)
Digital.CSIC. Repositorio Institucional del CSIC
PLoS ONE
PLoS ONE, Vol 12, Iss 3, p e0174454 (2017)
Popis: 18 p.-4 fig.-6 tab. López, María et al.
Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little is known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms in seven clinical isolates of A. baumannii. Microarray analysis of one of these clinical isolates, Ab1 (A. baumannii ST-2_clon_2010), previously cultured in the presence of 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ enzyme (α/ß hydrolase gene, AidA). This QQ enzyme was present in all non-motile clinical isolates (67% of which were isolated from the respiratory tract) cultured in nutrient depleted LB medium. Interestingly, this gene was not located in the genome of the only motile clinical strain growing in this medium (A. baumannii strain Ab421_GEIH-2010 [Ab7], isolated from a blood sample). The AidA protein expressed in E. coli showed QQ activity. Finally, we observed downregulation of the AidA protein (QQ system attenuation) in the presence of H2O2 (ROS stress). In conclusion, most of the A. baumannii clinical strains were not surface motile (84%) and were of respiratory origin (67%). Only the pilT gene was involved in surface motility and related to the QS system. Finally, a new QQ enzyme (α/ß hydrolase gene, AidA protein) was detected in these strains.
This study was funded by grant PI13/02390 awarded to MT within the State Plan for R+D+I 2013-2016 (National Plan for Scientific Research, Technological Development and Innovation 2008-2011) and co-financed by the ISCIII-Deputy General Directorate of evaluation and Promotion of Research - European Regional Development Fund "A way of Making Europe" and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015).
Databáze: OpenAIRE
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