Exocytosis and mobility of histamine vesicles exhibit no difference to that of histamine-negative vesicles in guinea pig superior cervical ganglion neurons
| Autor: | Gonghao He, Teng Li, Jia Meng, Jing Hu, Xiao-Xing Luo, Min Jia, Yuan-Qiang Zhang, Xue Ma |
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| Rok vydání: | 2011 |
| Předmět: |
Superior cervical ganglion
Sympathetic nervous system General Neuroscience Vesicle Guinea Pigs Presynaptic Terminals Superior Cervical Ganglion Biology Axonal Transport Histamine Release Synaptic vesicle Exocytosis chemistry.chemical_compound medicine.anatomical_structure chemistry Immunology Biophysics medicine Animals Synaptic Vesicles Neuron Neurotransmitter Cells Cultured Histamine |
| Zdroj: | NeuroReport. 22:274-281 |
| ISSN: | 0959-4965 |
| Popis: | Our previous studies have shown that histamine existed widely in the sympathetic nervous system and functioned differentially on the sympathetic nerve activation level. Therefore, in this study, we tried to find out whether it is the special exocytosis/recycling of histamine-containing vesicles that contribute to those differential histamine synaptic effects. By using N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridinium dibromide and histamine immunostaining methods, we confirmed that histamine was stored in small vesicles and found that the histamine-containing vesicles included the recycling pool and the reserve pool. However, we also found for the first time that the release and mobility kinetics of histamine-containing vesicles were identical to that of histamine-negative vesicles. In conclusion, these findings provide further characters of histamine as a sympathetic neurotransmitter. |
| Databáze: | OpenAIRE |
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