In vitro metabolism and toxicity assessment of toxin T-514 (Peroxisomicine A1) of Karwinskia humboldtiana in microsomes and primary cultured hepatocytes
| Autor: | Alfredo Piñeyro-López, V. Rivas, Lourdes Garza-Ocañas, Noemí Waksman, Magdalena Gómez-Silva |
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| Rok vydání: | 2005 |
| Předmět: |
Magnetic Resonance Spectroscopy
Guinea Pigs Toxicology medicine.disease_cause Ultraviolet therapy Mass Spectrometry Mice Dogs Species Specificity Karwinskia humboldtiana medicine Animals Humans Cytotoxic T cell Cytotoxicity Cells Cultured Chromatography High Pressure Liquid Anthracenes Dose-Response Relationship Drug biology Karwinskia Cytotoxins Toxin Haplorhini General Medicine biology.organism_classification Rats Hep G2 Biochemistry Hepatocytes Microsomes Liver Microsome Ultraviolet Therapy Drugs Chinese Herbal |
| Zdroj: | Toxicology in Vitro. 19:47-53 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2004.06.006 |
| Popis: | T-514 (Peroxisomicine A(1)) from Karwinskia humboldtiana is a dimeric hydroxyanthracenone with a highly selective cytotoxic effect on tumor cells. We evaluated the metabolism of this compound in two in vitro systems (liver microsomes and hepatocytes) and assessed the cytotoxicity of its metabolites on normal and tumor cells. Microsomes (12.5, 125 and 250 microg of protein/ml) and hepatocytes (1 x 10(6) cells/ml) were incubated with the toxin (25 microM) for 0.5, 1, 3, 6, 9, 12 and 24 h and the samples were examined using chromatographic analysis and UV spectra. Two metabolites (M1 and M2) were detected in the rat microsomes and one (M1) in the monkey microsomes. The retention times and UV spectra of the peaks were very similar to those of the toxin T-514. M1 was isolated and identified as a mixture of two isomers. The cytotoxicity of the metabolites was evaluated in Chang liver and Hep G2 cells but they did not show the selective cytotoxic effect on tumor cells seen in the original compound. |
| Databáze: | OpenAIRE |
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