Involvement of structurally distinct cupuassu chitinases and osmotin in plant resistance to the fungus Moniliophthora perniciosa

Autor: Karina Peres Gramacho, Fabienne Micheli, Rafael Moysés Alves, Lucilia Helena Marcellino, Raner José Santana Silva
Přispěvatelé: Universidade Estadual De Santa Cruz [Brazil] (UESC), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Cocoa Research Center CEPEC-CEPLAC, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)DTE0038/2013, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
[SDV]Life Sciences [q-bio]
Moniliophthora
Chitin
Plant Science
01 natural sciences
Moniliophthora perniciosa
F30 - Génétique et amélioration des plantes
chemistry.chemical_compound
Arabidopsis
Witches' broom disease
Expression des gènes
Chitosanase
Disease Resistance
Plant Proteins
2. Zero hunger
biology
Chitinases
food and beverages
Pathogenesis-related proteins
Molecular Docking Simulation
Molecular docking
Brazil
maladie du balai de sorcière
Pouvoir pathogène
Theobroma grandiflorum [EN]
Chitine
Microbiology
03 medical and health sciences
Chitosane
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
H20 - Maladies des plantes
Chitosan
Cacao
Abiotic stress
fungi
biology.organism_classification
030104 developmental biology
chemistry
Chitinase
biology.protein
Theobroma grandiflorum
Gene expression
Agaricales
010606 plant biology & botany
Zdroj: Plant Physiology and Biochemistry
Plant Physiology and Biochemistry, Elsevier, 2020, 148, pp.142-151. ⟨10.1016/j.plaphy.2020.01.009⟩
ISSN: 1873-2690
0981-9428
DOI: 10.1016/j.plaphy.2020.01.009⟩
Popis: International audience; The cupuassu tree (Theobroma grandiflorwn) is a crop of great economic importance to Brazil, mainly for its pulp and seeds, which are used in food industry. However, cupuassu fruit production is threatened by witches' broom disease caused by the fungus Moniliophthora perniciosa. As elements of its defense mechanisms, the plant can produce and accumulate pathogenesis-related (PR) proteins such as chitinases and osmotins. Here, we identified three cupuassu PR proteins (TgPR3, TgPR5 and TgPR8) from cupuassu-M. perniciosa interaction RNA-seq data. TgPR3 and TgPR8 corresponded to chitinases, and TgPR5 to osmotin; they are phylogenetically related to cacao and to Arabidopsis PR sequences involved in biotic and abiotic stress. The TgPR proteins' tridimensional structure was obtained through homology modeling, and molecular docking with chitin and chitosan showed that the TgPR proteins can interact with both cell wall molecules and presented a higher affinity for chitosan. TgPR gene expression was analyzed by RT-qPCR on resistant and susceptible cupuassu genotypes infected by M. perniciosa at 8, 24, 48 and 72 h after infection (hai). The TgPR genes showed higher expression in resistant plants compared to the susceptible ones, mainly for TgPR5 at 8 and 24 hai, while the expression was lower in the susceptible cupuassu plants. To our knowledge, this is the first in silico and in vitro reports of cupuassu PR protein. The data suggested that TgPRs could be involved in recognizing mechanisms of the plant's innate immune system through chitin receptors. Our results also suggest a putative role of chitinase/chitosanase for the TgPR5/osmotin.
Databáze: OpenAIRE
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