DNA Repair Biomarker for Lung Cancer Risk and its Correlation With Airway Cells Gene Expression
Autor: | Bruce A.J. Ponder, Tamar Paz-Elizur, Ronen Fluss, Martin O'Reilly, Robert C. Rintoul, Federico M. Giorgi, Laurence S. Freedman, Hyunjin Kim, Zvi Livneh, Yael Leitner-Dagan, Barak Markus, Yentl Evgy, Kerstin B. Meyer |
---|---|
Přispěvatelé: | Paz-Elizur, Tamar, Leitner-Dagan, Yael, Meyer, Kerstin B, Markus, Barak, Giorgi, Federico M, O'Reilly, Martin, Kim, Hyunjin, Evgy, Yentl, Fluss, Ronen, Freedman, Laurence S, Rintoul, Robert C, Ponder, Bruce, Livneh, Zvi, Rintoul, Robert [0000-0003-3875-3780], Apollo - University of Cambridge Repository |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Oncology
Cancer Research medicine.medical_specialty DNA repair Population 32 Biomedical and Clinical Sciences Article Transcriptome 03 medical and health sciences 0302 clinical medicine Clinical Research DNA Damage Repair Internal medicine Genetics medicine Risk factor Lung cancer education Lung Cancer 030304 developmental biology 0303 health sciences education.field_of_study business.industry Prevention 3 Good Health and Well Being Odds ratio 3211 Oncology and Carcinogenesis medicine.disease 4.1 Discovery and preclinical testing of markers and technologies 3. Good health 030220 oncology & carcinogenesis Respiratory Biomarker (medicine) 4.4 Population screening 4 Detection screening and diagnosis business 4.2 Evaluation of markers and technologies |
Zdroj: | JNCI Cancer Spectrum |
ISSN: | 2515-5091 |
Popis: | Background Improving lung cancer risk assessment is required because current early-detection screening criteria miss most cases. We therefore examined the utility for lung cancer risk assessment of a DNA Repair score obtained from OGG1, MPG, and APE1 blood tests. In addition, we examined the relationship between the level of DNA repair and global gene expression. Methods We conducted a blinded case-control study with 150 non–small cell lung cancer case patients and 143 control individuals. DNA Repair activity was measured in peripheral blood mononuclear cells, and the transcriptome of nasal and bronchial cells was determined by RNA sequencing. A combined DNA Repair score was formed using logistic regression, and its correlation with disease was assessed using cross-validation; correlation of expression to DNA Repair was analyzed using Gene Ontology enrichment. Results DNA Repair score was lower in case patients than in control individuals, regardless of the case’s disease stage. Individuals at the lowest tertile of DNA Repair score had an increased risk of lung cancer compared to individuals at the highest tertile, with an odds ratio (OR) of 7.2 (95% confidence interval [CI] = 3.0 to 17.5; P Conclusions The DNA Repair score, previously shown to be a lung cancer risk factor in the Israeli population, was validated in this independent study as a mechanism-based cancer risk biomarker and can substantially improve current lung cancer risk prediction, assisting prevention and early detection by computed tomography scanning. |
Databáze: | OpenAIRE |
Externí odkaz: |