Depletion and fractionation technologies in plasma proteomic analysis
| Autor: | Douglas Hinerfeld, Sun W. Tam, John Pirro |
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| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | Expert Review of Proteomics. 1:411-420 |
| ISSN: | 1744-8387 1478-9450 |
| Popis: | This review intends to survey the traditional and current technologies in the depletion and subfractionation of plasma proteins for further analyses. The value of depletion aims to enrich low-abundant proteins by removing highly abundant proteins, such as albumin or immunoglobulin G, from plasma. With this approach, one can examine both the resulting high- and low-abundant protein fractions. The depleted protein population can be further subfractionated based on their isoelectric point ranges, creating a more discrete pool of proteins for detailed post-translational modification studies by methods such as 2D gel electrophoresis and mass spectrometry. The concept of divide to conquer will greatly enhance our ability to identify and characterize low-abundant proteins and cleaved peptides from plasma as important diagnostic markers or potential drug targets. This can potentially reverse the decline in the development of new plasma diagnostic tests. |
| Databáze: | OpenAIRE |
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