In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae
| Autor: | Ying Wang, Hui-Yu Gao, Hong-Tao Jin, Cai-Xia Qiu, Shuang-Cheng Ma, Hong-Ying Li, Guang Hu, Feng Wei, Wan-Fang Li, Jianbo Yang, Qi Wang, Yun-Fei Song, Xian-Long Cheng, Shu-Ting Wang, Yue Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Polygonum Emodin Drug Evaluation Preclinical Aspartate transaminase Traditional Chinese medicine RM1-950 Pharmacology [Trans]-emodin-emodin dianthrone 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Animals Yolk sac Medicine Chinese Traditional Zebrafish Zebrafish hepatotoxicity model biology Chemistry Plant Extracts Albumin Polyphenols General Medicine biology.organism_classification Dianthrone 030104 developmental biology medicine.anatomical_structure Polyphenol Chrysophanol 030220 oncology & carcinogenesis Larva biology.protein Fallopia multiflora Emodin-8-O-β-D-glucopyranoside Therapeutics. Pharmacology Chemical and Drug Induced Liver Injury |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 131, Iss, Pp 110524-(2020) |
| ISSN: | 1950-6007 |
| Popis: | Polygonum multiflorum Thunb. (PM) is a traditional Chinese medicine, commonly used to treat a variety of diseases. However, the hepatotoxicity associated with PM hampers its clinical application and development. In this study, we refined the zebrafish hepatotoxicity model with regard to the following endpoints: liver size, liver gray value, and the area of yolk sac. The levels of alanine aminotransferase, aspartate transaminase, albumin, and microRNAs-122 were evaluated to verify the model. Subsequently, this model was used to screen different extracts, components, and constituents of PM, including 70 % EtOH extracts of PM, four fractions from macroporous resin (components A, B, C, and D), and 19 compounds from component D. We found that emodin, chrysophanol, emodin-8-O-β-D-glucopyranoside, (cis)-emodin-emodin dianthrones, and (trans)-emodin-emodin dianthrones showed higher hepatotoxicity compared to other components in PM, whereas polyphenols showed lower hepatotoxicity. To the best of our knowledge, this study is the first to identify that dianthrones may account for the hepatotoxicity of PM. We believe that these findings will be helpful in regulating the hepatotoxicity of PM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect