Optimization of cDNA Amplification of Apricot Latent Virus (ApLV) from Various Plant Tissues Sources
| Autor: | Mustafa Gümüş, İsmail Can Paylan, H. M. Sipahioglu, Semih Erkan |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2007 |
| Předmět: |
Optimization
DNA Complementary RT-PCR Computational biology Biology law.invention ApLV law Complementary DNA Gene Polymerase chain reaction DNA Primers Reverse Transcriptase Polymerase Chain Reaction ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS Apricot latent virus Plants Virology Reverse transcriptase ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS ComputingMethodologies_PATTERNRECOGNITION Specific primers Flexiviridae InformationSystems_MISCELLANEOUS Nucleic Acid Amplification Techniques Agronomy and Crop Science Taq DNA Polymerase |
| Zdroj: | Pakistan Journal of Biological Sciences. 10:936-940 |
| ISSN: | 1028-8880 |
| Popis: | PubMed ID: 19069893 Although the reverse transcriptase polymerase chain reaction (RT-PCR) procedure is basically simple operation, often it is not possible to achieve optimum results without optimizing the protocols. An RT-PCR method targeting a 200 bp sequence of the CP gene of Apricot Latent Virus (ApLV) was used as a model to improve the detection limit and to compare the behavior of three different plant tissues in a RT-PCR assay. A number of factors should be considered when selecting the optimal system for RT-PCR. Important considerations include the optimal concentrations of MgCl2, dNTP, Taq DNA polymerase enzyme, specific primer and the amount of cDNA for the downstream applications. This study therefore discusses a series of critical PCR parameters and feasible strategies for optimization of RT-PCR detection of ApLV. © 2007 Asian Network for Scientific Information. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|