Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans

Autor: Huazong Zeng, Guobing Zhong, Dongjing Yan, Wangwei Cai, Guankui Du, Man Xiao
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Hemolytic anemia
Protein metabolism
lcsh:Medicine
medicine.disease_cause
Biochemistry
chemistry.chemical_compound
Mice
0302 clinical medicine
Drug Metabolism
Metabolites
Medicine and Health Sciences
lcsh:Science
Protein Metabolism
Mice
Inbred C3H
Multidisciplinary
Chemistry
Fatty Acids
food and beverages
Lipids
Vicia faba
Blood
Liver
030220 oncology & carcinogenesis
Urea cycle
Creatinine
Metabolic Pathways
Research Article
medicine.medical_specialty
Linoleic acid
Gas Chromatography-Mass Spectrometry
Linoleic Acid
03 medical and health sciences
Internal medicine
medicine
Animals
Metabolomics
Pharmacokinetics
Pharmacology
lcsh:R
Biology and Life Sciences
Cell Biology
medicine.disease
Oleic acid
Metabolic pathway
Oxidative Stress
030104 developmental biology
Endocrinology
Metabolism
lcsh:Q
Chromatography
Thin Layer
Oxidative stress
Drug metabolism
Biomarkers
Oleic Acid
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0151103 (2016)
ISSN: 1932-6203
Popis: Favism is a life-threatening hemolytic anemia resulting from the intake of fava beans by susceptible individuals with low erythrocytic glucose 6-phosphate dehydrogenase (G6PD) activity. However, little is known about the metabolomic changes in plasma and liver after the intake of fava beans in G6PD normal and deficient states. In this study, gas chromatography/mass spectrometry was used to analyze the plasma and liver metabolic alterations underlying the effects of fava beans in C3H- and G6PD-deficient (G6PDx) mice, and to find potential biomarkers and metabolic changes associated with favism. Our results showed that fava beans induced oxidative stress in both C3H and G6PDx mice. Significantly, metabolomic differences were observed in plasma and liver between the control and fava bean treated groups of both C3H and G6PDx mice. The levels of 7 and 21 metabolites in plasma showed significant differences between C3H-control (C3H-C)- and C3H fava beans-treated (C3H-FB) mice, and G6PDx-control (G6PDx-C)- and G6PDx fava beans-treated (G6PDx-FB) mice, respectively. Similarly, the levels of 7 and 25 metabolites in the liver showed significant differences between C3H and C3H-FB, and G6PDx and G6PDx-FB, respectively. The levels of oleic acid, linoleic acid, and creatinine were significantly increased in the plasma of both C3H-FB and G6PDx-FB mice. In the liver, more metabolic alterations were observed in G6PDx-FB mice than in C3H-FB mice, and were involved in a sugar, fatty acids, amino acids, cholesterol biosynthesis, the urea cycle, and the nucleotide metabolic pathway. These findings suggest that oleic acid, linoleic acid, and creatinine may be potential biomarkers of the response to fava beans in C3H and G6PDx mice and therefore that oleic acid and linoleic acid may be involved in oxidative stress induced by fava beans. This study demonstrates that G6PD activity in mice can affect their metabolic pathways in response to fava beans.
Databáze: OpenAIRE
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