Efficacy of phage therapy in pigs: systematic review and meta-analysis
| Autor: | Karina Desiree, Paul D. Ebner, Sabrina Mosimann |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Phage therapy Swine medicine.drug_class medicine.medical_treatment Animal Health and Well Being 030231 tropical medicine Antibiotics Microbiology Bacteriophage Feces 03 medical and health sciences 0302 clinical medicine systematic review phage Genetics medicine Animals Bacteriophages Treatment effect Phage Therapy Cecum Featured Collection Swine Diseases biology Inoculation General Medicine biology.organism_classification meta-analysis 030104 developmental biology Meta-analysis AcademicSubjects/SCI00960 Animal Science and Zoology Bacteria Food Science |
| Zdroj: | Journal of Animal Science |
| ISSN: | 1525-3163 0021-8812 |
| Popis: | Limits on the use and efficacy of various antibiotics coupled with negative consumer perception of the practice have together spurred substantial research into compounds that could reduce the use antibiotics to control bacterial diseases in pigs. Bacteriophages are often among such potential compounds, and various groups have examined the efficacy of bacteriophages or bacteriophage products in limiting transmission or colonization of targeted bacteria. The study presented here provides a systematic review of such studies followed by a meta-analysis of aggregated data produced by each study. The data set was limited to inputs (n = 19; 576 total observations) from studies where: 1) live pigs were inoculated with a known quantity of challenge bacteria; 2) challenged animals were treated with a known quantity of phages; 3) concentrations of the challenge bacteria were measured in different tissues/fluids following phage treatment; and 4) SD (or SE to allow calculation of SD) was reported. Concentrations of challenge bacteria were significantly lower in phage-treated pigs versus challenged but untreated pigs (P < 0.0001; effect size = −1.06 1log10 colony-forming units [CFU]/g). The effect size of phage treatment was significantly greater (P < 0.05) in samples collected 48 to 96 h following phage treatment versus those collected ≤ 24 h following phage treatment. Likewise, effect size of phage treatment was significantly greater in piglets versus market-weight pigs. Across observations, phage treatment effect sizes were greatest (P < 0.01) in fecal samples versus ileal or cecal samples. Taken together, these data indicate that phage treatment can significantly reduce the concentrations of targeted bacteria in pigs; scenarios exist, however, where phage treatment could predictably be more or less effective. |
| Databáze: | OpenAIRE |
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