Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis
Autor: | Peng Liang, Terrence Grady, Craig D. Logsdon, Stephen A. Ernst |
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Rok vydání: | 1997 |
Předmět: |
Chemokine
medicine.medical_specialty Pancreatic disease Pyrrolidines Time Factors Fluorescent Antibody Technique Gene Expression chemistry.chemical_compound Pyrrolidine dithiocarbamate Acinus Thiocarbamates Internal medicine medicine Animals Northern blot RNA Messenger Pancreas Chemokine CCL2 Differential display Hepatology biology Gastroenterology NF-kappa B medicine.disease Molecular biology Rats Chemokine CXCL10 Endocrinology medicine.anatomical_structure chemistry Pancreatitis Acute Disease biology.protein Acute pancreatitis Cytokines Chemokines Chemokines CXC |
Zdroj: | Gastroenterology. 113(6) |
ISSN: | 0016-5085 |
Popis: | BACKGROUND & AIMS: The molecular mechanisms underlying pancreatitis are largely unknown. The goal of this study was to identify an early genetic event that correlated with pancreatitis. METHODS: Differential display of messenger RNAs (mRNAs) was conducted on normal pancreas vs. those of animals with secretagogue-induced pancreatitis. Northern blots from normal animals and animals with experimental acute pancreatitis were probed with cloned complementary DNAs for chemokines. Pancreatitis was induced with cerulein and by retrograde injection of bile salts. Immunocytochemistry was used to identify the source of chemokine expression. Pyrrolidine dithiocarbamate was tested for effects on chemokine expression and pancreatitis. RESULTS: A differentially amplified band was consistently observed early after cerulein hyperstimulation. This band was identified as a portion of the mob-1 gene, an alpha-chemokine. Northern analysis indicated that mRNAs for mob-1 and another chemokine, mcp-1, were induced after cerulein hyperstimulation in vivo. mob-1 mRNA was also induced by retrograde injection of bile salts and by cerulein in acinar cells in vitro. mob-1 protein was localized to exocrine cells in pancreata of diseased animals. Pyrrolidine dithiocarbamate inhibited both chemokine gene expression and early inflammatory characteristics of pancreatitis. CONCLUSIONS: Chemokines are induced in acinar cells by treatments that induce pancreatitis and may play an important role in the early stages of the disease. (Gastroenterology 1997 Dec;113(6):1966-75) |
Databáze: | OpenAIRE |
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