Manipulation of pre and postnatal androgen environments and anogenital distance in rats
| Autor: | Dária Louise Barbosa Machado, Anderson Joel Martino-Andrade, Katlyn Barp Meyer, Chris Gennings, Shanna H. Swan, Amanda Caroline Venturelli, Diogo Henrique Kita, Rafaella Adams, Rosana Nogueira de Morais |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male endocrine system medicine.medical_specialty medicine.drug_class Toxicology Flutamide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Internal medicine Diethylhexyl Phthalate Testis medicine Weaning Animals Testosterone 030219 obstetrics & reproductive medicine Dose-Response Relationship Drug business.industry Anogenital distance Phthalate Pregnancy Outcome Androgen Antagonists Organ Size Androgen Spermatids Rats Disease Models Animal 030104 developmental biology Endocrinology chemistry In utero Prenatal Exposure Delayed Effects Androgens Gestation Female business Penis |
| Zdroj: | Toxicology. |
| ISSN: | 1879-3185 |
| Popis: | We examined the anogenital distance (AGD) plasticity in rats through the manipulation of the androgen environment in utero and during puberty. Dams were treated from gestation days 13–20 with vehicle, flutamide (20 mg/kg/day), di-(2-ethylhexyl) phthalate (DEHP, 750 mg/kg/day), or testosterone (1.0 mg/kg/day). After weaning, male pups were randomly assigned to one of four postnatal groups, which received the same treatments given prenatally. Sixteen treatment groups were established based on the combination of pre- and postnatal exposures. The postnatal treatments were conducted from postnatal days 23–53. In utero flutamide and DEHP exposure significantly shortened male AGD, although this effect was more pronounced in flutamide-exposed rats. Postnatal flutamide, DEHP, and testosterone induced slight but significant reductions in male AGD. Our study indicates that AGD is a stable anatomical landmark that reflects the androgen action in utero , although it can also be slightly responsive to changes in the androgen environment following pubertal exposure. |
| Databáze: | OpenAIRE |
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