First Human Isolate of Hantavirus (Andes virus) in the Americas
| Autor: | Eliecer Villagra, Hector Galeno, Jorge Fernández, Eugenio Ramírez, Judith Mora, Jury Hernandez, Gregory J. Mertz |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Microbiology (medical) Orthohantavirus Time Factors Epidemiology Hantavirus Infections viruses Andes virus Fluorescent Antibody Technique lcsh:Medicine Hantavirus Pulmonary Syndrome Biology Immunofluorescence Virus lcsh:Infectious and parasitic diseases Evolution Molecular Fatal Outcome Chlorocebus aethiops medicine Animals Humans lcsh:RC109-216 Chile Child Vero Cells Hantavirus Hantavirus pulmonary syndrome medicine.diagnostic_test Research lcsh:R Middle Aged Virology Infectious Diseases Vero cell biology.protein Female Antibody Hantavirus Infection |
| Zdroj: | Emerging Infectious Diseases, Vol 8, Iss 7, Pp 657-661 (2002) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| DOI: | 10.3201/eid0807.010277 |
| Popis: | We isolated Andes virus (formal name: Andes virus [ANDV], a species in the genus Hantavirus), from serum of an asymptomatic 10-year-old Chilean boy who died 6 days later of hantavirus pulmonary syndrome (HPS). The serum was obtained 12 days after his grandmother died from HPS and 2 days before he became febrile. No hantavirus immunoglobulin (Ig) G or IgM antibodies were detected in the serum sample. After three blind passages, ANDV antigens were detected in Vero E6 cells by immunofluorescence assay and enzyme-linked immunosorbent assay, and ANDV RNA was detected by reverse transcription-polymerase chain reaction. A fragment of the virus genome showed 96.2% nucleotide identity with that of prototype ANDV. To our knowledge, this is the first isolation of any agent of hemorrhagic fever with renal syndrome from a human and the first such isolation of hantavirus before symptoms of that syndrome or HPS began. |
| Databáze: | OpenAIRE |
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