Human Cytomegalovirus DNA among Women with Breast Cancer
| Autor: | Nilofar Nisi, Nastaran Ranjbari, Shahram Jalilian, Azarakhsh Azaran, Alireza Samarbafzadeh, Kimia Makvandi, Peyman Sepahvand, Manoochehr Makvandi, Abdulhasan Talaei-Zadeh, Roya Pirmoradi, Kambiz Ahmadi Angali |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Human cytomegalovirus medicine.medical_specialty Adenoma Cytomegalovirus Breast Neoplasms ductal carcinoma Iran Real-Time Polymerase Chain Reaction medicine.disease_cause Polymerase Chain Reaction Gastroenterology law.invention 03 medical and health sciences breast cancer 0302 clinical medicine Breast cancer law Internal medicine medicine Humans Polymerase chain reaction business.industry Incidence Carcinoma Ductal Breast General Medicine Middle Aged Ductal carcinoma Prognosis medicine.disease Fibroadenoma Ductal Breast Carcinoma 030104 developmental biology Case-Control Studies 030220 oncology & carcinogenesis Cytomegalovirus Infections DNA Viral Female Carcinogenesis business Follow-Up Studies Research Article |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| Popis: | Section Title Breast cancer is the most common cause of death among women worldwide. Although there are many known risk factors in breast cancer development, infectious diseases have appeared as one of the important key to contribute to carcinogenesis formation. The effects of Human Cytomegalovirus (HCMV) on women with breast cancer has been recently studied and reported. To contribute to this research trend, this study was conducted to evaluate the association between HCMV and the women with breast cancer. Objective: This experiment aimed to evaluate HCMV DNA in women with breast cancer in Ahvaz city, Iran. Materials and Methods: A total of 37 formalin fixed paraffin embedded tissues of the patients with ductal breast carcinoma and 35 paraffin embedded tissues of the patients with fibro adenoma as control group were collected. The deparaffinization of all the samples were carried out and the DNA was extracted. Initially, the PCR test was carried out to detect beta –globulin DNA as an internal control. For those samples positive for beta –globulin DNA, Polymerase Chain reaction (PCR) was used to detect HCMV for the tests and control samples. Results: Among 37 ductal breast carcinoma, 20 (54.04%) cases were proved positive for HCMV DNA by PCR. While among the 35 control group (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028). The prevalences of HCMV DNA among the age groups 30-39, 40-49 and >50 years were 7 (72.22%), 9 (69.23%), 4 (57.14%), respectively (P=0.066). A high frequency of HCMV DNA was detected in tumor grade III, 13/18 (58.33%) compared with tumor grade II, 7/19 (36.84%) (p=0.044). A high frequency of 16/24 (66.66%) of HCMV DNA was found in invasive ductal breast cancer compared with 4/13 (30.76%) HCMV DNA in situ (P |
| Databáze: | OpenAIRE |
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