Generation of 275.4-nm UV output from a large-frame argon-ion laser for fluorescence detection in capillary electrophoresis

Autor: Steven J. Kok, Gerard Ph. Hoornweg, Ton de Ridder, Cees Gooijer, Udo A. Th. Brinkman, Nel H. Velthorst
Přispěvatelé: BioAnalytical Chemistry, Chemistry and Pharmaceutical Sciences
Jazyk: angličtina
Rok vydání: 1998
Předmět:
Zdroj: Journal of Chromatography A, 806, 355-360. Elsevier
Kok, S J, de Ridder, T, Brinkman, U A T, Velthorst, N H, Gooijer, C & Hoornweg, G P 1998, ' Generation of 275.4-nm UV output from a large-frame argon-ion laser for fluorescence detection in capillary electrophoresis. ', Journal of Chromatography A, vol. 806, pp. 355-360 . https://doi.org/10.1016/S0021-9673(98)00078-8
ISSN: 1873-3778
0021-9673
Popis: A standard, relatively old, large-frame argon-ion laser, which is available in many laboratories, was modified to produce output in the deep-UV (275–306 nm) region by installing a set of inexpensive, commercially available laser mirrors. The deep-UV output is generally applicable as excitation wavelength in the field of biochemical and environmental analysis. However, it has received little attention in the literature, probably because a complete laser set-up normally requires a high investment. The modification described, which includes a thorough cleaning of the Brewster windows of the laser tube and a careful alignment of the laser, is a good alternative to this investment. In daily operation, only some minor realignments are required. As an illustration of the applicability, excitation with the 275.4-nm line was used for laser-induced detection of native fluorescent aromatic sulphonates in capillary electrophoresis.
Databáze: OpenAIRE
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