Temperature Derivative Spectroscopy To Monitor the Autoxidation Decay of Cytochromes P450

Autor:	Ilia G. Denisov, Abhinav Luthra, Stephen G. Sligar
Rok vydání:	2011
Předmět:	Hemeprotein Autoxidation Chemistry Spectrum Analysis Protein dynamics Kinetics Relaxation (NMR) Temperature Analytical chemistry Activation energy Article Analytical Chemistry Reaction rate Cytochrome P-450 Enzyme System Humans Spectroscopy Oxidation-Reduction
Zdroj:	Analytical Chemistry. 83:5394-5399
ISSN:	1520-6882 0003-2700
Popis:	Temperature derivative spectroscopy (TDS), a type of relaxation spectroscopy, is a powerful tool to study protein dynamics (Berendzen, J.; Braunstein, D. Proc. Natl. Acad. Sci. U. S. A. 1990, 87, 1). We developed the version of temperature derivative spectroscopy to monitor kinetics of autoxidation of cytochromes P450 and applied it to study the properties of the oxy-ferrous complex of a human membrane bound P450, CYP19A1 (aromatase), and that of a bacterial soluble P450, CYP101 when bound with their most common substrates, androstenedione (AD) and camphor, respectively. TDS extends the panel of methods that can be used to monitor heme protein kinetics, providing a rapid measurement technique and enabling measurement of the autoxidation rate over a wide range of temperatures, yielding the activation energy as well as absolute reaction rate in a single experiment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b40119b6282a5f3467fcd521eb7733c8 https://doi.org/10.1021/ac2009349 Zobrazit plný text záznamu