Induction of duodenal mucosal tumors of intestinal epithelial cell origin showing frequent nuclear β-catenin accumulation similar to the concurrently induced colorectal tumors in rats after treatment with azoxymethane
| Autor: | Yumi Kangawa, Makoto Shibutani, Toshinori Yoshida, Sayaka Mizukami, Masayuki Kimura, Nobuhiko Onda, Yoh Kikuchihara |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Carcinogenesis Duodenum Azoxymethane Adenocarcinoma Histogenesis Toxicology Gastroenterology Pathology and Forensic Medicine chemistry.chemical_compound Cytokeratin Duodenal Neoplasms Internal medicine Animals Medicine Intestinal Mucosa beta Catenin MUC1 business.industry Mucin Cell Biology General Medicine Rats Inbred F344 Rats chemistry Tumor progression Catenin Carcinogens Cancer research Immunohistochemistry Colorectal Neoplasms business |
| Zdroj: | Experimental and Toxicologic Pathology. 67:349-353 |
| ISSN: | 0940-2993 |
| DOI: | 10.1016/j.etp.2015.03.002 |
| Popis: | Azoxymethane (AOM) is a potent carcinogen used for induction of colon tumors in rats and mice. It is also known that AOM treatment induces small bowel tumors in addition to colorectal tumors in rats. The present study examined the histogenesis of AOM-induced rat duodenal tumors in comparison with concurrently induced colorectal tumors by histochemical and immunohistochemical approaches. Duodenal and colorectal tumors were positive for both periodic acid-Schiff reaction and Alcian blue staining. Immunohistochemically, duodenal tumors were positive for intestinal epithelial markers such as cytokeratin (CK) 20 (100%) and mucin (MUC) 2 (91.7%) but negative for pancreaticobiliary markers such as CK7 (100%) and MUC1 (100%). All colorectal tumors were also negative for CK7 and MUC1 but positive for CK20. Eighty percent of colorectal tumors were positive for MUC2. In addition, nuclear accumulation of β-catenin was found in duodenal tumors (70.8%), which was similar to colorectal tumors (90.0%). These results indicate that duodenal tumors induced by AOM treatment of rats were derived from intestinal epithelium. Similar to colorectal tumors, nuclear accumulation of β-catenin indicates activation of Wnt signaling as a driving force for tumor progression in AOM-induced duodenal tumors. |
| Databáze: | OpenAIRE |
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