Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods

Autor:	Yan Zhang, Jiaqi Shi, Honglei Li, Shuo Wang, Zeping Shao
Rok vydání:	2019
Předmět:	Hot Temperature Pharmaceutical Science 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 Cartridge chemistry.chemical_compound 0404 agricultural biotechnology lcsh:Organic chemistry Limit of Detection 5-hydroxymethyl-2-furfural Chromatography detector Drug Discovery Furaldehyde Physical and Theoretical Chemistry Detection limit Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Extraction (chemistry) 04 agricultural and veterinary sciences Repeatability 040401 food science 0104 chemical sciences Chemistry (miscellaneous) Acrylamide acrylamide Molecular Medicine simultaneous detection Methanol hplc-dad Food Analysis Hplc dad
Zdroj:	Molecules, Vol 24, Iss 20, p 3734 (2019) Molecules Volume 24 Issue 20
ISSN:	1420-3049
DOI:	10.3390/molecules24203734
Popis:	Acrylamide and 5-hydroxymethyl-2-furfural (5-HMF) are two of the most abundant compounds generated during thermal processing. A simple method for the simultaneous quantitation of acrylamide and 5-HMF was developed and successfully applied in thermally processed foods. Acrylamide and 5-HMF were co-extracted with methanol and then purified and enriched by an Oasis HLB solid-phase extraction cartridge, simultaneously analyzed by high-performance liquid chromatography and detected with a diode array detector, respectively, at their optimal wavelength. The linear concentration range was found to be 25&ndash 5000 &mu g/L with high linear correlation coefficients (R > 0.999). The limit of detection and the limit of quantitation for acrylamide and 5-HMF were 6.90 &mu g/L and 4.66 &mu g/L, and 20.90 &mu g/L and 14.12 &mu g/L, respectively. The recovery of acrylamide and 5-HMF in biscuits, bread, Chinese doughnuts, breakfast cereals, and milk-based baby foods was achieved at 87.72&ndash 96.70% and 85.68&ndash 96.17% with RSD at 0.78&ndash 3.35% and 0.55&ndash 2.81%, respectively. The established method presents simplicity, accuracy and good repeatability, and can be used for the rapid simultaneous quantitation of acrylamide and 5-HMF in thermally processed foods.
Databáze:	OpenAIRE
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