Deletion of thePDR16gene influences the plasma membrane properties of the yeastKluyveromyces lactis
| Autor: | Dana Gášková, Marcela Morvová, Yvetta Gbelska, Peter Griac, Iva Jančíková, Nora Toth Hervay, Zuzana Šimová, Eduard Goffa, Alexandra Svrbicka, L. Šikurová |
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| Rok vydání: | 2015 |
| Předmět: |
Kluyveromyces lactis
Vesicle-associated membrane protein 8 First line Cell Membrane Immunology Cell General Medicine Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Yeast Fungal Proteins Multiple drug resistance Kluyveromyces Membrane medicine.anatomical_structure Biochemistry Genetics medicine Phospholipid Transfer Proteins Molecular Biology Gene Gene Deletion |
| Zdroj: | Canadian Journal of Microbiology. 61:273-279 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/cjm-2014-0627 |
| Popis: | The plasma membrane is the first line of cell defense against changes in external environment, thus its integrity and functionality are of utmost importance. The plasma membrane properties depend on both its protein and lipid composition. The PDR16 gene is involved in the control of Kluyveromyces lactis susceptibility to drugs and alkali metal cations. It encodes the homologue of the major K. lactis phosphatidylinositol transfer protein Sec14p. Sec14p participates in protein secretion, regulation of lipid synthesis, and turnover in vivo. We report here that the plasma membrane of the Klpdr16Δ mutant is hyperpolarized and its fluidity is lower than that of the parental strain. In addition, protoplasts prepared from the Klpdr16Δ cells display decreased stability when subjected to hypo-osmotic conditions. These changes in membrane properties lead to an accumulation of radiolabeled fluconazole and lithium cations inside mutant cells. Our results point to the fact that the PDR16 gene of K. lactis (KlPDR16) influences the plasma membrane properties in K. lactis that lead to subsequent changes in susceptibility to a broad range of xenobiotics. |
| Databáze: | OpenAIRE |
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