Can phase masks extend depth-of-field in localization microscopy?
| Autor: | Antony Lee, Caroline Kulcsár, Pierre Bon, Hervé Sauer, Laurent Cognet, Olivier Lévêque, François Goudail |
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| Přispěvatelé: | Laboratoire Charles Fabry / Imagerie et Information, Laboratoire Charles Fabry (LCF), Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Photonique, Numérique et Nanosciences (LP2N), Université de Bordeaux (UB)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), International Society for Optics and Photonics |
| Rok vydání: | 2020 |
| Předmět: |
Physics
Point spread function [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] Microscope business.industry Phase (waves) Image processing 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences law.invention 010309 optics Optical axis Optics law 0103 physical sciences Microscopy Depth of field 0210 nano-technology business [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing Wavefront coding |
| Zdroj: | Unconventional Optical Imaging II Unconventional Optical Imaging II, International Society for Optics and Photonics, Apr 2020, Online Only, France. pp.50-58, ⟨10.1117/12.2558426⟩ |
| DOI: | 10.1117/12.2558426 |
| Popis: | International audience; In localization microscopy, the position of isolated fluorescent emitters are estimated with a resolution better than the diffraction limit. In order to image thick samples, which are common in biological applications, there is considerable interest in extending the depth-of-field of such microscopes in order to make their accuracy as invariant as possible to defocus. For that purpose, we propose to optimize annular binary phase masks placed in the pupil of the microscope in order to generate a point spread function for which the localization accuracy is almost invariant along the optical axis. The optimization criterion is defined as the localization accuracy in the plane expressed in terms of the Cramér-Rao bound. We show that the optimal masks significantly increase the depth-of-field of single-molecule imaging techniques relatively to an usual microscope objective. |
| Databáze: | OpenAIRE |
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