Replisome Localization in Vegetative and Aerial Hyphae of Streptomyces coelicolor
| Autor: | Beata Ruban-Ośmiałowska, Jolanta Zakrzewska-Czerwińska, Aleksandra Smulczyk-Krawczyszyn, Keith F. Chater, Dagmara Jakimowicz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
DNA Replication
Hypha Macromolecular Substances Recombinant Fusion Proteins Green Fluorescent Proteins dnaN Genetics and Molecular Biology Streptomyces coelicolor DNA-Directed DNA Polymerase Microbiology chemistry.chemical_compound Bacterial Proteins Molecular Biology Mycelium biology fungi DNA replication Chromosomes Bacterial biology.organism_classification Subcellular localization Cell biology chemistry Microscopy Fluorescence Replisome DNA |
| Popis: | Using a functional fusion of DnaN to enhanced green fluorescent protein, we examined the subcellular localization of the replisome machinery in the vegetative mycelium and aerial mycelium of the multinucleoid organism Streptomyces coelicolor . Chromosome replication took place in many compartments of both types of hypha, with the apical compartments of the aerial mycelium exhibiting the highest replication activity. Within a single compartment, the number of “current” ongoing DNA replications was lower than the expected chromosome number, and the appearance of fluorescent foci was often heterogeneous, indicating that this process is asynchronous within compartments and that only selected chromosomes undergo replication. |
| Databáze: | OpenAIRE |
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