Protein malnutrition prevents heat conservation induced by amino acid infusion during general anesthesia in rats
| Autor: | Toshiyasu Suzuki, Mariko Watanabe, Masahiro Kanazawa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Endocrinology Diabetes and Metabolism medicine.medical_treatment Muscle Proteins 030209 endocrinology & metabolism Hypothermia Anesthesia General Body Temperature Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Endocrinology medicine Animals Insulin Amino Acids Phosphorylation Muscle Skeletal Saline chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Kinase TOR Serine-Threonine Kinases Malnutrition Intracellular Signaling Peptides and Proteins Skeletal muscle Thermogenesis Amino acid Enzyme medicine.anatomical_structure chemistry Protein Biosynthesis Anesthesia Dietary Proteins medicine.symptom Proto-Oncogene Proteins c-akt |
| Zdroj: | Nutrition Research. 65:79-88 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/j.nutres.2019.02.007 |
| Popis: | The intravenous administration of an amino acid (AA) mixture during general anesthesia reduces anesthesia-induced hypothermia. AA-induced skeletal muscle protein synthesis and thermogenesis play important roles in the antihypothermic effects of AAs. We hypothesized that a preanesthetic dietary protein deficiency impairs the antihypothermic effects of AAs during general anesthesia due to a reduction in thermogenesis caused by a decrease in muscle protein synthesis. Sprague-Dawley rats were divided into 4 groups: fed a control diet plus saline (CON-SAL) or the AA mixture (CON-AA), and fed a protein-free diet plus saline (PF-SAL) or the AA mixture (PF-AA). SAL solution or AA mixture solution was infused for 180 minutes during sevoflurane anesthesia, and rectal temperatures were measured. Rectal temperatures were significantly higher in the CON-AA group than in the PF-AA group 90 to 180 minutes after initiating the intravenous infusion of the test solutions. There was no significant difference between the PF-SAL and PF-AA groups. Plasma insulin concentrations were significantly higher in the CON-AA group than in the PF-AA group (P .05). The phosphorylation states of protein kinase B, mammalian target of rapamycin, and eukaryotic initiation factor 4E-binding protein 1 were significantly greater in the CON-AA group than in the PF-AA group (P .05, P .05, and P .01, respectively). Our results indicated that a dietary protein deficiency before general anesthesia impaired the antihypothermic effects of an AA mixture infusion during general anesthesia by decreasing muscle protein synthesis through the insulin-stimulated phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin complex 1 signaling pathway followed by metabolic heat production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect