Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number
| Autor: | Shinichiro Nakamura, Fumihito Koizumi, Hisashi Wada, Kazuhiro Nouso, Yasushi Shiratori, Yuji Noguchi, Tetsuya Nakada, Ryo Tanaka, Eiichi Nakayama, Kazuhiko Nakagawa, Shuichiro Sato, Yao-Tseng Chen, Achim Jungbluth, Shoichiro Fujita, Kosei Hasegawa, Toshiro Ono, Lloyd J. Old |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Cancer Research Placenta Biology Antigens Neoplasm Neoplasms Testis medicine Humans Tissue Distribution RNA Messenger Messenger RNA Oncogene Reverse Transcriptase Polymerase Chain Reaction Ovary Cancer Antibodies Monoclonal Membrane Proteins medicine.disease Molecular biology Reverse transcription polymerase chain reaction Oncology Liver Protein Biosynthesis Cancer/testis antigens Immunohistochemistry Female NY-ESO-1 Liver cancer |
| Zdroj: | International journal of oncology. 26(1) |
| ISSN: | 1019-6439 |
| Popis: | We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA. |
| Databáze: | OpenAIRE |
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