Genetically engineered, live, attenuated vaccines protect nonhuman primates against aerosol challenge with a virulent IE strain of Venezuelan equine encephalitis virus
Autor: | Matthew G. Lackemeyer, Cathleen M. Lind, Douglas S. Reed, Lawrence J. Sullivan, Michael D. Parker, William D. Pratt |
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Rok vydání: | 2005 |
Předmět: |
Male
Fever viruses Virulence Enzyme-Linked Immunosorbent Assay Viral Plaque Assay Alphavirus Antibodies Viral Vaccines Attenuated medicine.disease_cause Virus Body Temperature Microbiology Encephalitis Virus Venezuelan Equine Lymphopenia medicine Animals Aerosols Vaccines Synthetic Attenuated vaccine General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Encephalomyelitis Venezuelan Equine Viral Vaccines biology.organism_classification Virology Vaccination Macaca fascicularis Infectious Diseases Togaviridae Venezuelan equine encephalitis virus biology.protein Molecular Medicine Female Antibody |
Zdroj: | Vaccine. 23:3139-3147 |
ISSN: | 0264-410X |
DOI: | 10.1016/j.vaccine.2004.12.023 |
Popis: | Two live, attenuated strains of Venezuelan equine encephalitis virus (VEE), IE1150K and V3526, were administered to macaques to determine if they could elicit protection against an aerosol challenge with virulent VEE virus of the IE variety (VEEV-IE). These viruses were rescued from full-length cDNA clones of 68U201 (VEEV-IE variety) and Trinidad donkey (VEEV-IA/B variety), respectively, and both have a furin cleavage site deletion mutation and a second-site resuscitating mutation. Both vaccines elicited neutralizing antibodies to viruses of the homologous variety but not to viruses of the heterologous variety. Eight weeks after vaccination, the macaques were challenged by aerosol exposure to virulent 68U201. Macaques vaccinated with V3526 were protected as well as macaques inoculated with IE1009, the wild-type infectious clone of 68U201. However, IE1150K failed to significantly protect macaques relative to controls. V3526 has now been shown to protect macaques against both IA/B [Pratt WD, Davis NL, Johnston RE, Smith JF. Genetically engineered, live attenuated vaccines for Venezuelan equine encephalitis: testing in animal models. Vaccine 2003;21(25–26):3854–62] and IE strains of VEE viruses. |
Databáze: | OpenAIRE |
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