Neutrophil activation after percutaneous transluminal coronary angioplasty
| Autor: | Hironori Toshima, Takafumi Ueno, Kohji Hiyamuta, Kazunori Kuwano, Hisao Ikeda, Tameo Oda, Yoshinori Koga, Akihiro Yamaga, Masayoshi Yoh, Hiroshi Nakayama |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Integrins Neutrophils medicine.medical_treatment Myocardial Ischemia Macrophage-1 Antigen Coronary Disease Coronary Angiography Neutrophil Activation Coronary artery disease chemistry.chemical_compound Dichlorofluorescein In vivo Angioplasty Internal medicine medicine Humans cardiovascular diseases Angioplasty Balloon Coronary Coronary sinus Aged biology Pancreatic Elastase business.industry Cell Membrane Hydrogen Peroxide Middle Aged medicine.disease Flow Cytometry surgical procedures operative chemistry Integrin alpha M Neutrophil elastase CD18 Antigens Cardiology biology.protein Female Cardiology and Cardiovascular Medicine business Leukocyte Elastase Ex vivo |
| Zdroj: | American heart journal. 128(6 Pt 1) |
| ISSN: | 0002-8703 |
| Popis: | We investigated whether percutaneous transluminal coronary angioplasty (PTCA) would induce neutrophil activation in patients with coronary artery disease. Blood samples were taken from the coronary sinus in 14 patients who underwent PTCA and in 9 control subjects who underwent coronary arteriography (CAG). Flow cytometry was used to measure membrane surface expression of beta 2 integrin (CD11b) and the generation of hydrogen peroxide in neutrophils after ex vivo phorbol myristate acetate stimulation by 2,'7'-dichlorofluorescein. Neutrophil elastase was measured by an immunoenzymatic method. Surface expression of CD11b increased significantly, approximately twofold, after PTCA but not after CAG. Mean fluorescence intensity of 2',7'-dichlorofluorescein in stimulated neutrophils decreased significantly after PTCA, suggesting a previous in vivo activation, but not after CAG. Neutrophil elastase increased significantly after PTCA but not after CAG. These data indicate that PTCA induces neutrophil activation and suggest that neutrophils may contribute to the ischemic injury. |
| Databáze: | OpenAIRE |
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