Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP
| Autor: | Irene Frangiadaki, Eftihia Xylouri, Aleka Tzimogianni, Michael Arabatzis, Afroditi Milioni, Aristea Velegraki, George Arsenis |
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| Rok vydání: | 2006 |
| Předmět: |
General Veterinary
Sequence analysis Arthrodermataceae Biology Dermatomycosis medicine.disease_cause Molecular biology Polymerase Chain Reaction law.invention Restriction site Arthroderma law Dermatophyte medicine Animals Dermatomycoses Rabbits Internal transcribed spacer Restriction fragment length polymorphism Polymerase chain reaction Polymorphism Restriction Fragment Length Skin |
| Zdroj: | Veterinary dermatology. 17(5) |
| ISSN: | 0959-4493 |
| Popis: | Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding DdeI restriction profiles of the ITS1/4 PCR products matched one of the two published T. mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct DdeI restriction sites for Arthroderma benhamiae and A. vanbreuseghemii, while all DdeI-generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification. |
| Databáze: | OpenAIRE |
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